重组Anti-PGK1抗体[EPR19057] (ab199438)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19057] to PGK1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-PGK1抗体[EPR19057]
参阅全部 PGK1 一抗 -
描述
兔单克隆抗体[EPR19057] to PGK1 -
宿主
Rabbit -
特异性
ab199438 has weak cross-reactivity with PGK2
-
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: HeLa, HepG2, Jurkat, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain and fetal heart lysates; Mouse brain, heart and kidney lysates; Rat brain, heart and kidney lysates. ICC/IF: HeLa and Jurkat cells. Flow Cyt (intra): HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19057 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab199438于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/150.
|
|
WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
|
ICC/IF |
1/250.
|
说明 |
---|
Flow Cyt (Intra)
1/150. |
WB
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
ICC/IF
1/250. |
靶标
-
功能
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. -
疾病相关
Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations. -
序列相似性
Belongs to the phosphoglycerate kinase family. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 5230 Human
- Entrez Gene: 18655 Mouse
- Entrez Gene: 24644 Rat
- Omim: 311800 Human
- SwissProt: P00558 Human
- SwissProt: P09411 Mouse
- SwissProt: P16617 Rat
- Unigene: 78771 Human
see all -
别名
- Cell migration-inducing gene 10 protein antibody
- Epididymis secretory sperm binding protein Li 68p antibody
- HEL S 68p antibody
see all
图片
-
Lanes 1-2 : Anti-PGK1 antibody [EPR19057] (ab199438) at 1/1000 dilution
Lanes 3-4 : Anti-IL-21R antibody [RM0109-7F27] (ab86742) at 1/5000 dilution
Lanes 1 & 3 : PGK1 Human Recombinant Protein
Lanes 2 & 4 : PGK2 Human Recombinant Protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Exposure time: 5 secondsBlocking and Diluting Buffer: 5% NFDM /TBST
Lanes 3 and 4 Exposure: 60 seconds
-
All lanes : Anti-PGK1 antibody [EPR19057] (ab199438) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
-
Immunofluorescent analysis of 100% methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PGK1 with ab199438 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining in HeLa cells. The nuclear counterstain is DAPI (blue).
-
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PGK1 with ab199438 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
-
Immunofluorescent analysis of 100% methanol-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling PGK1 with ab199438 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining in Jurkat cells. The nuclear counterstain is DAPI (blue).
-
All lanes : Anti-PGK1 antibody [EPR19057] (ab199438) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-PGK1 antibody [EPR19057] (ab199438) at 1/2000 dilution
Lane 1 : Mouse brain lysate at 10 µg
Lane 2 : Mouse heart lysate at 10 µg
Lane 3 : Mouse kidney lysate at 10 µg
Lane 4 : Rat brain lysate at 10 µg
Lane 5 : Rat heart lysate at 10 µg
Lane 6 : Rat kidney lysate at 10 µg
Lane 7 : C6 (Rat glial tumor cells) whole cell lysate
Lane 8 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 10 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (11)
ab199438 被引用在 11 文献中.
- Pan X et al. LncRNA HCG18 promotes osteosarcoma growth by enhanced aerobic glycolysis via the miR-365a-3p/PGK1 axis. Cell Mol Biol Lett 27:5 (2022). PubMed: 34991445
- Li JQ et al. Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro. Stem Cells Int 2022:4363632 (2022). PubMed: 35069747
- Yagi M et al. Mitochondrial translation deficiency impairs NAD+ -mediated lysosomal acidification. EMBO J 40:e105268 (2021). PubMed: 33528041
- Ma X et al. circAGFG1 sponges miR-28-5p to promote non-small-cell lung cancer progression through modulating HIF-1a level. Open Med (Wars) 16:703-717 (2021). PubMed: 34013042
- Luo X et al. LHX9, a p53-binding protein, inhibits the progression of glioma by suppressing glycolysis. Aging (Albany NY) 13:22109-22119 (2021). PubMed: 34536269