Anti-PGAM1抗体(ab2220)

Reviews (1)Q&A (1)References (6)
Western blot - Anti-PGAM1 antibody (ab2220)
  • Western blot - Anti-PGAM1 antibody (ab2220)

Key features and details

  • Goat polyclonal to PGAM1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

重组抗体的批间可重复性更强

Product image
Anti-PGAM1 antibody [EPR8042] (ab129191)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-PGAM1抗体
    参阅全部 PGAM1 一抗

  • 描述

    山羊多克隆抗体to PGAM1

  • 宿主

    Goat

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig, Monkey

  • 免疫原

    Synthetic peptide: KAMEAVAAQGKAKK, corresponding to C terminal amino acids 241-254 of Human PGAM1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    GenBank Accession Number – NP_002620.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab2220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa.

1 hour primary incubation is recommended for this product.

靶标

图片

  • Western blot - Anti-PGAM1 antibody (ab2220)
    Western blot - Anti-PGAM1 antibody (ab2220)
    Anti-PGAM1 antibody (ab2220) at 0.01 µg/ml + Human liver lysate at 35 µg

    Developed using the ECL technique.


    Primary incubated for 1 hour

  • Western blot - Anti-PGAM1 antibody (ab2220)
    Western blot - Anti-PGAM1 antibody (ab2220)
    ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

文献 (6)

发表研究结果有使用 ab2220？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2220 被引用在 6 文献中.

  • Okuda J  et al. Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice. PLoS One 8:e72173 (2013). WB ; Mouse . PubMed: 23951293
  • Hallows WC  et al. Regulation of Glycolytic Enzyme Phosphoglycerate Mutase-1 by Sirt1 Protein-mediated Deacetylation. J Biol Chem 287:3850-8 (2012). WB ; Human . PubMed: 22157007
  • Lei Y  et al. Proteomics Identification of ITGB3 as a Key Regulator in Reactive Oxygen Species-induced Migration and Invasion of Colorectal Cancer Cells. Mol Cell Proteomics 10:M110.005397 (2011). WB, IHC-P ; Human . PubMed: 21622897
  • Klein-Scory S  et al. Immunoscreening of the extracellular proteome of colorectal cancer cells. BMC Cancer 10:70 (2010). WB ; Human . PubMed: 20184735
  • Evans MJ  et al. Mechanistic and structural requirements for active site labeling of phosphoglycerate mutase by spiroepoxides. Mol Biosyst 3:495-506 (2007). PubMed: 17579775
  • Evans MJ  et al. Target discovery in small-molecule cell-based screens by in situ proteome reactivity profiling. Nat Biotechnol 23:1303-7 (2005). PubMed: 16200062

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Western blot abreview for Anti-PGAM1 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (BRAIN)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
BRAIN
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Nov 16 2015

Question

I wonder about specificity of that antibody. Does it really recognize only PGAM1 and not PGAM2 form?

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Answer

Thank you for your enquiry. I have blasted the immunizing peptide sequence and found that 13 out of　14 amino acids were identical to the sequence of PGAM2. Therefore, it is likely that this product might cross react with PGAM2. However, since we have not tested it in our laboratory, we are not sure of the results. I hope this information will be helpful. Should you require anything else, please do not hesitate to contact me.

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