Anti-Pescadillo抗体(ab88543)
Key features and details
- Mouse polyclonal to Pescadillo
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Pescadillo抗体
参阅全部 Pescadillo 一抗 -
描述
小鼠多克隆抗体to Pescadillo -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant full length protein within Human Pescadillo. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- WB: PC-12 and A-431 cell lysates; Pescadillo transfected 293T cell lysate ICC/IF: HeLa cell
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88543于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. -
组织特异性
Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin. -
序列相似性
Belongs to the pescadillo family.
Contains 1 BRCT domain. -
翻译后修饰
Sumoylated. -
细胞定位
Nucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli. - Information by UniProt
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数据库链接
- Entrez Gene: 23481 Human
- Entrez Gene: 289740 Rat
- Omim: 605819 Human
- SwissProt: O00541 Human
- SwissProt: Q3B8N8 Rat
- Unigene: 517543 Human
- Unigene: 9521 Rat
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别名
- FLJ43790 antibody
- Homo sapiens pescadillo mRNA complete cds antibody
- PES antibody
see all
图片
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Anti-Pescadillo antibody (ab88543) at 1 µg/ml + PC-12 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
Anti-Pescadillo antibody (ab88543) at 1 µg/ml + A-431 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Pescadillo antibody (ab88543) at 1 µg/ml
Lane 1 : Pescadillo transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
ab88543, at 10 µg/ml, staining Pescadillo in HeLa cell by Immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab88543 被引用在 3 文献中.
- Xu KD et al. Licochalcone A inhibits cell growth through the downregulation of the Hippo pathway via PES1 in cholangiocarcinoma cells. Environ Toxicol 37:564-573 (2022). PubMed: 34845814
- Chen B et al. The Long Noncoding RNA CCAT2 Induces Chromosomal Instability Through BOP1-AURKB Signaling. Gastroenterology 159:2146-2162.e33 (2020). PubMed: 32805281
- Nakaguro M et al. Nucleolar protein PES1 is a marker of neuroblastoma outcome and is associated with neuroblastoma differentiation. Cancer Sci 106:237-43 (2015). ICC . PubMed: 25557119