Anti-Peroxiredoxin 6抗体(ab59543)


  • 产品名称
    Anti-Peroxiredoxin 6抗体
    参阅全部 Peroxiredoxin 6 一抗
  • 描述
    兔多克隆抗体to Peroxiredoxin 6
  • 宿主
  • 特异性
    ab59543 reacts with Peroxiredoxin 6.
  • 经测试应用
    适用于: Flow Cyt, WB, ELISA, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein (Rat) Peroxiredoxin 6

  • 阳性对照
    • Rat and mouse lung and human brain.
  • 常规说明
    Staining pattern: cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. Stains human brain astrocytes in Parkinson's and Alzheimer's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies.



Our Abpromise guarantee covers the use of ab59543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/4000. Predicted molecular weight: 25 kDa.
ELISA 1/1000 - 1/4000.
IHC-Fr 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.


  • 功能
    Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
  • 序列相似性
    Belongs to the ahpC/TSA family. Rehydrin subfamily.
    Contains 1 thioredoxin domain.
  • 细胞定位
    Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 Cys antibody
    • 1 Cys peroxiredoxin antibody
    • 1 Cys PRX antibody
    • 1 cysPrx antibody
    • 1-Cys peroxiredoxin antibody
    • 1-Cys PRX antibody
    • 24 kDa protein antibody
    • 9430088D19Rik antibody
    • AA690119 antibody
    • Acidic calcium independent phospholipase A2 antibody
    • Acidic calcium-independent phospholipase A2 antibody
    • aiPLA2 antibody
    • Antioxidant protein 2 antibody
    • AOP2 antibody
    • Aop2 rs3 antibody
    • Brp 12 antibody
    • Ciliary body glutathione peroxidase antibody
    • CP 3 antibody
    • EC antibody
    • EC antibody
    • EC 3.1.1. antibody
    • Epididymis secretory sperm binding protein Li 128m antibody
    • GPx antibody
    • HEL S 128m antibody
    • KIAA0106 antibody
    • Liver 2D page spot 40 antibody
    • Ltw4 antibody
    • Lvtw 4 antibody
    • MGC46173 antibody
    • mKIAA0106 antibody
    • Non selenium glutathione peroxidase antibody
    • Non-selenium glutathione peroxidase antibody
    • NSGPx antibody
    • ORF06 antibody
    • OTTHUMP00000032693 antibody
    • p29 antibody
    • Peroxiredoxin-6 antibody
    • Peroxiredoxin6 antibody
    • PHGPx antibody
    • Phospholipase A2 lysosomal antibody
    • PLA2 antibody
    • PRDX 6 antibody
    • Prdx5 antibody
    • PRDX6 antibody
    • Prdx6 rs3 antibody
    • PRDX6_HUMAN antibody
    • PRX antibody
    • Red blood cells page spot 12 antibody
    • Thiol specific antioxidant protein antibody
    see all


  • Lane 1 : Anti-Peroxiredoxin 6 antibody (ab59543)
    Lane 2 : Anti-Peroxiredoxin 6 antibody (ab59543) at 1/1000 dilution

    All lanes : Mouse skeletal muscle whole tissue lysates

    Lysates/proteins at 20 µg per lane.

    All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Additional bands at: 25 kDa, 38, 45 kDa (possible non-specific binding). We are unsure as to the identity of these extra bands.

    Exposure time: 5 minutes

    See Abreview

  • ab59543 staining Peroxiredoxin 6 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview

  • ab59543 at 1/100 dilution staining Peroxiredoxin 6 in astrocytes in Parkinson's disease by Immunohistochemistry. Secondary antibody anti-rabbit IgG conjugated to Cy3 (1/100).
  • ab59543 at 1/1000 dilution staining Peroxiredoxin 6 in astrocytes in Parkinson's disease by IHC-P. Secondary antibody Donkey anti-rabbit conjugated to biotin.
  • All lanes : Anti-Peroxiredoxin 6 antibody (ab59543) at 1/2500 dilution

    Lane 1 : Wild-type rat cortex
    Lanes 2-5 : Transgenic (APP/PS1) male mouse cortical lysate
    Lanes 6-9 : Wild-type male mouse cortical lysate

    All lanes : Alexa Fluor 680-conjugated goat anti rabbit IgG polyclonal antibody

    Predicted band size: 25 kDa
    Observed band size: ~25 kDa (why is the actual band size different from the predicted?)

    Western blot showing detection of Peroxiredoxin 6 in mouse and rat cortical lysates by ab59543.

    See Abreview

  • ICC/IF image of ab59543 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59543, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab59543 staining Peroxiredoxin 6 in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

    See Abreview


This product has been referenced in:
  • Paul S  et al. STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. Redox Biol 14:423-438 (2018). WB ; Rat . Read more (PubMed: 29078168) »
  • Yumnam S  et al. Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array. Mol Med Rep 16:5386-5392 (2017). Read more (PubMed: 28849065) »

See all 16 Publications for this product


Thank you for your reply.

Blocking reagents also are subject tooptimization.

If your customer needs a strong blocking solution for their experiment, I can confirm that the combination of BSA and serum is optimal.

Glycine i...

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The particular blocking step your customer mentioned is our standard procedure for ICC/IF.
I have attached our extended standard protocol for IHC-P from our website to this email for her convenience.

As for the dilution range, this wi...

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Before proceeding, I am sorry I am not able to trace the order number provided, or an order from Italy on that date. I...

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Thank you for providing this further information.

I appreciate your cooperation and understand your concerns. It is regrettable the results have not been successful.

In order to help with our investigation of this case before deci...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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