Recombinant full length protein (Rat) Peroxiredoxin 6
Rat and mouse lung and human brain.
Staining pattern: cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. Stains human brain astrocytes in Parkinson's and Alzheimer's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Belongs to the ahpC/TSA family. Rehydrin subfamily. Contains 1 thioredoxin domain.
Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 6 antibody (ab59543)This image is courtesy of an Abreview submitted by Mahesh Shivananjappa
ab59543 staining Peroxiredoxin 6 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.
ICC/IF image of ab59543 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59543, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry - Anti-Peroxiredoxin 6 antibody (ab59543)Image courtesy of an anonymous Abreview.
ab59543 staining Peroxiredoxin 6 in Human platelet cells by Flow cytometry. Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.