Peroxidase Activity Assay试剂盒(ab155895)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
概述
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产品名称
Peroxidase Activity Assay试剂盒
参阅全部 HRP 试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids -
检测类型
Enzyme activity -
种属反应性
与反应: Mammals, Other species -
产品概述
Abcam’s Peroxidase Assay Kit (ab155895) provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the redfluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (λmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K772 Peroxidase Activity Colorimetric/Fluorometric Assay Kit. K772-100 is the same size as the 100 test size of ab155895.
Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer XVIII 1 x 25ml Developer Solution V 1 vial H2O2 Standard 1 x 0.1ml OxiRed Probe 1 x 0.2ml -
研究领域
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相关性
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. -
细胞定位
Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles. -
别名
- Horseradish Peroxidase
- HPRC1
- HRP
see all
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab155895 被引用在 2 文献中.
- Jang H et al. Suppressive Effect of Bioactive Extracts of Bacillus sp. H8-1 and Bacillus sp. K203 on Tomato Wilt Caused by Clavibacter michiganensis subsp. michiganensis. Microorganisms 10:N/A (2022). PubMed: 35208859
- Dos Reis VP et al. Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Bothrops jararacussu Venom and Isolated PLA2s. Biomed Res Int 2022:5266211 (2022). PubMed: 35872869