Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney cell line(HEK293))
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney cell line(HEK293)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ELC X-ray
Exposure
15 second(s)
Positive control
Mouse neuroblastoma(N2A) cell extract
Additional data
Additional Notes
Works great for mouse neuroblastoma cells but does not works for human kidney cells(HEK293)
Abcam response
We would like to thank this customer for submitting their review. Peripherin is primarily expressed in neurons so it may be expressed at undetectable levels in HEK293 cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 22 2016