Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney cell line(COS7))
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney cell line(COS7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ELC X-ray
Exposure
15 second(s)
Positive control
N2A(Mouse) cell extract
Additional data
Additional Notes
This antibody works excellent for mouse neuroblastoma cells
Abcam response
We appreciate this customer's review for using the antibody with monkey cell lysates. We had not tested the antibody with the species and were unsure of whether it would cross react. It performed as expected with the mouse N2A cell lysates (validated and guaranteed species).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 22 2016