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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments

Anti-Peripherin抗体(ab4666)

  • Datasheet
  • SDS
Reviews (18)Q&A (1)References (24)

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Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (ab4666)
  • Western blot - Anti-Peripherin antibody (ab4666)
  • Western blot - Anti-Peripherin antibody (ab4666)

Key features and details

  • Rabbit polyclonal to Peripherin
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Rabbit, Cat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Peripherin抗体
    参阅全部 Peripherin 一抗
  • 描述

    兔多克隆抗体to Peripherin
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Rabbit, Cat, Human
  • 免疫原

    Recombinant full length rat peripherin purified from E. coli.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液

    Preservative: 0.01% Sodium azide
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Peripherin
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Peripherin protein (ab114335)

应用

Our Abpromise guarantee covers the use of ab4666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Detects a band of approximately 57 kDa.

靶标

  • 功能

    Class-III neuronal intermediate filament protein.
  • 序列相似性

    Belongs to the intermediate filament family.
  • Target information above from: UniProt accession P41219 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5630 Human
    • Entrez Gene: 19132 Mouse
    • Entrez Gene: 24688 Rat
    • Omim: 170710 Human
    • SwissProt: P41219 Human
    • SwissProt: P15331 Mouse
    • SwissProt: P21807 Rat
    • Unigene: 37044 Human
    • Unigene: 2477 Mouse
    • Unigene: 11086 Rat
    see all
  • 别名

    • NEF 4 antibody
    • NEF4 antibody
    • Neurofilament 4 (57kD) antibody
    • Neurofilament 4 antibody
    • Perf antibody
    • PERI_HUMAN antibody
    • Peripherin antibody
    • PRPH 1 antibody
    • prph antibody
    • PRPH1 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (ab4666)
    Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (ab4666)

    Immunocytochemistry/Immunofluorescence analysis of mixed neuron/glia cultures from newborn rat brain labelling Peripherin (green) with ab4666 and Neurofilament heavy polypeptide (red) with ab4680. A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain Neurofilament heavy polypeptide and not peripherin. The blue channel reveals the localization of DNA.

  • Western blot - Anti-Peripherin antibody (ab4666)
    Western blot - Anti-Peripherin antibody (ab4666)
    Anti-Peripherin antibody (ab4666) at 1/1000 dilution + Rat spinal cord homogenate

    Observed band size: 57 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Peripherin antibody (ab4666)
    Western blot - Anti-Peripherin antibody (ab4666)
    Anti-Peripherin antibody (ab4666) at 1 µg/ml + Recombinant Human Peripherin protein (ab114335) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 20 seconds


    Secondary antibody - goat anti-rabbit HRP preasorbed (ab97080)

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (24)

    发表研究结果有使用 ab4666?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4666 被引用在 24 文献中.

    • Liu W  et al. Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction. Int J Biol Sci 16:85-98 (2020). PubMed: 31892848
    • Nickolls AR  et al. Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells. Cell Rep 30:932-946.e7 (2020). PubMed: 31968264
    • Saveri P  et al. Charcot-Marie-Tooth Type 2B: A New Phenotype Associated with a Novel RAB7A Mutation and Inhibited EGFR Degradation. Cells 9:N/A (2020). PubMed: 32326241
    • Yu H  et al. Neuroprotective effects of midazolam on focal cerebral ischemia in rats through anti-apoptotic mechanisms. Int J Mol Med 43:443-451 (2019). PubMed: 30431057
    • Huang CW  et al. Multipotent vascular stem cells contribute to neurovascular regeneration of peripheral nerve. Stem Cell Res Ther 10:234 (2019). PubMed: 31376835
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 19 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peripherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon adenocarcinoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 8.5 buffer
    Permeabilization
    No
    Specification
    Colon adenocarcinoma
    Fixative
    Formaldehyde
    Read More

    Mr. Allan Wang

    Verified customer

    提交于 Mar 20 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peripherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Oesophagus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Oesophagus
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 May 26 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Peripherin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (Kidney)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 May 23 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Peripherin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 02 2016

    Western blot abreview for Anti-Peripherin antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Kidney cell line(HEK293))
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Kidney cell line(HEK293)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 22 2016

    Western blot abreview for Anti-Peripherin antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Kidney cell line(COS7))
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Kidney cell line(COS7)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 22 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peripherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Dorsal root ganglia)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Dorsal root ganglia
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Demi Murray

    Verified customer

    提交于 Nov 24 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peripherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (Small intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Small intestine
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Nov 17 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peripherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Small intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Small intestine
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Nov 17 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peripherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Nov 17 2015

    1-10 of 19 Abreviews or Q&A

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