Anti-Peripherin抗体(ab4666)
Key features and details
- Rabbit polyclonal to Peripherin
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Peripherin抗体
参阅全部 Peripherin 一抗 -
描述
兔多克隆抗体to Peripherin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant full length rat peripherin purified from E. coli.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.01% Sodium azide -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
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| WB |
1/1000 - 1/2000. Detects a band of approximately 57 kDa.
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| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
1/1000 - 1/2000. Detects a band of approximately 57 kDa. |
靶标
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功能
Class-III neuronal intermediate filament protein. -
序列相似性
Belongs to the intermediate filament family. - Information by UniProt
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数据库链接
- Entrez Gene: 5630 Human
- Entrez Gene: 24688 Rat
- Omim: 170710 Human
- SwissProt: P41219 Human
- SwissProt: P21807 Rat
- Unigene: 37044 Human
- Unigene: 11086 Rat
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别名
- NEF 4 antibody
- NEF4 antibody
- Neurofilament 4 (57kD) antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (ab4666)
Immunocytochemistry/Immunofluorescence analysis of mixed neuron/glia cultures from newborn rat brain labelling Peripherin (green) with ab4666 and Neurofilament heavy polypeptide (red) with ab4680. A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain Neurofilament heavy polypeptide and not peripherin. The blue channel reveals the localization of DNA.
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Western blot - Anti-Peripherin antibody (ab4666)Anti-Peripherin antibody (ab4666) at 1/1000 dilution + Rat spinal cord homogenate
Observed band size: 57 kDa why is the actual band size different from the predicted? -
Western blot - Anti-Peripherin antibody (ab4666)Anti-Peripherin antibody (ab4666) at 1 µg/ml +Recombinant Human Peripherin protein (ab114335) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 secondsSecondary antibody - goat anti-rabbit HRP preasorbed (ab97080)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab4666 被引用在 26 文献中.
- Liu W et al. Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction. Int J Biol Sci 16:85-98 (2020). PubMed: 31892848
- Nickolls AR et al. Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells. Cell Rep 30:932-946.e7 (2020). PubMed: 31968264
- Saveri P et al. Charcot-Marie-Tooth Type 2B: A New Phenotype Associated with a Novel RAB7A Mutation and Inhibited EGFR Degradation. Cells 9:N/A (2020). PubMed: 32326241
- Li J et al. CDGSH Iron Sulfur Domain 2 Deficiency Inhibits Cell Proliferation and Induces Cell Differentiation of Neuroblastoma. Pathol Oncol Res 26:1725-1733 (2020). PubMed: 31642036
- Liang Y et al. iTRAQ-based proteomic analysis discovers potential biomarkers of diffuse axonal injury in rats. Brain Res Bull 153:289-304 (2019). PubMed: 31539556