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Recombinant full length protein corresponding to Rat Peripherin.
Our Abpromise guarantee covers the use of ab24524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/20000. Detects a band of approximately 57 kDa.|
Immunofluorescent analysis of mixed fibroblast and PC-12 (rat adrenal gland pheochromocytoma cell line) pheochromocytoma cell culture stained for Peripherin using ab24524 (green) at a 1/5000 dilution and co-stained with rabbit anti-vimentin (red) at a 1/2000 dilution. The blue is DAPI staining of nuclear DNA.
ab24524 reveals cytoplasmic filamentous staining in the small PC-12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.
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