Anti-Periostin抗体(ab152099)
Key features and details
- Rabbit polyclonal to Periostin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Periostin抗体
参阅全部 Periostin 一抗 -
描述
兔多克隆抗体to Periostin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Pig -
免疫原
Recombinant fragment, corresponding to a region within amino acids 604-807 of Human Periostin (Q15063).
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阳性对照
- A431, H1299, HeLa S3 and NIH 3T3 whole cell lysates; HeLa cells; Human gastric tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab152099于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 93 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 93 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
组织特异性
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
序列相似性
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
翻译后修饰
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 10631 Human
- Entrez Gene: 50706 Mouse
- Omim: 608777 Human
- SwissProt: Q15063 Human
- SwissProt: Q62009 Mouse
- Unigene: 136348 Human
- Unigene: 721018 Human
- Unigene: 236067 Mouse
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别名
- Fasciclin-I like antibody
- MGC119510 antibody
- MGC119511 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human gastric tissue labeling Periostin with ab152099 at 1/100 dilution.
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Anti-Periostin antibody (ab152099) at 1/1000 dilution + H1299 whole cell
lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE -
Anti-Periostin antibody (ab152099) at 1/1000 dilution + NIH 3T3 whole cell
lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Periostin with ab152099 at 1/200. Lower image is merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab152099 被引用在 3 文献中.
- Li X et al. Growth differentiation factor 11 mitigates cardiac radiotoxicity via activating AMPKa. Free Radic Res 55:176-185 (2021). PubMed: 33557626
- Zhang J et al. Integrative Analysis Reveals a miRNA-mRNA Regulatory Network and Potential Causative Agents in the Asthmatic Airway Epithelium. J Asthma Allergy 14:1307-1321 (2021). PubMed: 34744440
- Huang YW et al. A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder. Aging Dis 9:1043-1057 (2018). PubMed: 30574417