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Cardiovascular Lipids / Lipoproteins Adipose Related Lipid Droplet Protein

Anti-Perilipin A+B抗体(ab3527)

  • Datasheet
Submit a review Submit a question References (2)

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Western blot - Anti-Perilipin A+B antibody (ab3527)

    Key features and details

    • Rabbit polyclonal to Perilipin A+B
    • Suitable for: WB
    • Reacts with: Mouse, Rat
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Perilipin A+B抗体
    • 描述

      兔多克隆抗体to Perilipin A+B
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Rat
    • 免疫原

      Synthetic peptide corresponding to Rat Perilipin A+B aa 1-17 (N terminal).
      Sequence:

      MSMNKGPTLLDGDLPEQ


      (Peptide available as ab5010)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • 纯度

      IgG fraction
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cardiovascular
      • Lipids / Lipoproteins
      • Adipose Related
      • Lipid Droplet Protein
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Atherosclerosis
      • Lipoprotein metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Types of disease
      • Cancer

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    Our Abpromise guarantee covers the use of ab3527 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/1000.

    靶标

    • 相关性

      Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.
    • 细胞定位

      Lipid droplet
    • 数据库链接

      • Entrez Gene: 103968 Mouse
      • Entrez Gene: 25629 Rat
      • SwissProt: Q8CGN5 Mouse
      • SwissProt: P43884 Rat
      • 别名

        • Lipid droplet associated protein antibody
        • PERIA antibody
        • PERIB antibody
        • Perilipin A antibody
        • Perilipin antibody
        • Perilipin B antibody
        • PLIN antibody
        see all

      图片

      • Western blot - Anti-Perilipin A+B antibody (ab3527)
        Western blot - Anti-Perilipin A+B antibody (ab3527)
        Anti-Perilipin A+B antibody (ab3527) + Cell extracts prepared from 3T3-L1 cells


        Western blot detection of perilipin A and B in 3T3-L1 cell extract using ab3527.

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
    • 文献 (2)

      发表研究结果有使用 ab3527?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3527 被引用在 2 文献中.

      • Matushansky I  et al. A developmental model of sarcomagenesis defines a differentiation-based classification for liposarcomas. Am J Pathol 172:1069-80 (2008). WB ; Human . PubMed: 18310505
      • Fernández-Veledo S  et al. Hyperinsulinemia induces insulin resistance on glucose and lipid metabolism in a human adipocytic cell line: paracrine interaction with myocytes. J Clin Endocrinol Metab 93:2866-76 (2008). PubMed: 18430774

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      There are currently no Customer reviews or Questions for ab3527.
      Please use the links above to contact us or submit feedback about this product.

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