PE Anti-Integrin alpha 6抗体[GoH3] (ab95703)
Key features and details
- PE Rat monoclonal [GoH3] to Integrin alpha 6
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
PE Anti-Integrin alpha 6抗体[GoH3]
参阅全部 Integrin alpha 6 一抗 -
描述
PE大鼠单克隆抗体[GoH3] to Integrin alpha 6 -
宿主
Rat -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Integrin alpha 6. Mouse mammary tumor cells
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阳性对照
- Normal Human peripheral blood cells/leukocytes
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常规说明
The GoH3 antibody has been reported to have functional activity in blocking the binding of Integrin alpha 6 to laminin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
The GoH3 antibody has been reported to have functional activity in blocking the binding of Integrin alpha 6 to laminin. -
克隆
单克隆 -
克隆编号
GoH3 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95703于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 0.06-0.125µg for 105-8 cells.
Staining the cell sample in a final volume of 100 µl is recommended.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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说明 |
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Flow Cyt
Use 0.06-0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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靶标
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功能
Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). -
组织特异性
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). -
疾病相关
Epidermolysis bullosa letalis, with pyloric atresia -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
翻译后修饰
Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
Palmitoylation by DHHC3 enhances stability and cell surface expression. -
细胞定位
Cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3655 Human
- Entrez Gene: 16403 Mouse
- Omim: 147556 Human
- SwissProt: P23229 Human
- SwissProt: Q61739 Mouse
- Unigene: 133397 Human
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别名
- Alpha6p antibody
- CD49 antigen-like family member F antibody
- CD49f antibody
see all
图片
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Flow Cytometry analysis of normal human peripheral blood cells stained with Rat IgG2a kappa Isotype Control Phycoerythrin (blue histogram) or Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) (ab95703) (purple histogram). Cells in the lymphocyte gate were used for analysis.
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Flow cytometry staining of normal Human peripheral blood cells with 0.06 µg of Rat IgG2a Isotype Control PE (open histogram) or 0.06 µg of ab95703 (filled histogram). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.
实验方案
数据表及文件
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Datasheet download
文献 (11)
ab95703 被引用在 11 文献中.
- Gerdes P et al. Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells. Nat Commun 13:7470 (2022). PubMed: 36463236
- Wang C et al. Elevated level of mitochondrial reactive oxygen species via fatty acid ß-oxidation in cancer stem cells promotes cancer metastasis by inducing epithelial-mesenchymal transition. Stem Cell Res Ther 10:175 (2019). PubMed: 31196164
- Lin X et al. Increased expression of TMED2 is an unfavorable prognostic factor in patients with breast cancer. Cancer Manag Res 11:2203-2214 (2019). PubMed: 31114314
- Krishna S et al. Dynamic expression of tRNA-derived small RNAs define cellular states. EMBO Rep 20:e47789 (2019). PubMed: 31267708
- Shabani R et al. Elimination of mouse tumor cells from neonate spermatogonial cells utilizing cisplatin-entrapped folic acid-conjugated poly(lactic-co-glycolic acid) nanoparticles in vitro. Int J Nanomedicine 13:2943-2954 (2018). PubMed: 29849458