PE Anti-CD7抗体[Huly-m2] (ab1160)
Key features and details
- PE Mouse monoclonal [Huly-m2] to CD7
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
概述
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产品名称
PE Anti-CD7抗体[Huly-m2]
参阅全部 CD7 一抗 -
描述
PE小鼠单克隆抗体[Huly-m2] to CD7 -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
This antibody does not react with granulocytes and B lymphocytes. -
种属反应性
与反应: Human -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
克隆
单克隆 -
克隆编号
Huly-m2 -
骨髓瘤
unknown -
同种型
IgG2a -
轻链类型
unknown -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
应用说明
Diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL) andAcute myeloid leukaemia (CD7+ AML)in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
For FACS:
Add 100ml of whole blood to a staining tube.
To this tube add 10ml of CD7 (PE)reagent.
Mix gently and incubate 20-30 minutes at 2-8°C.
Add 2ml of BIO LISANTE(ab1159) at room temperature.
Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
Wash cells two timeswith 3ml of PBS.
Resuspend cells in 0.5-1ml of PBS and analyse.
CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
For FACS:
Add 100ml of whole blood to a staining tube.
To this tube add 10ml of CD7 (PE)reagent.
Mix gently and incubate 20-30 minutes at 2-8°C.
Add 2ml of BIO LISANTE(ab1159) at room temperature.
Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
Wash cells two timeswith 3ml of PBS.
Resuspend cells in 0.5-1ml of PBS and analyse.
CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
靶标
-
功能
Not yet known. -
序列相似性
Contains 1 Ig-like (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 924 Human
- Omim: 186820 Human
- SwissProt: P09564 Human
- Unigene: 36972 Human
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别名
- CD7 antibody
- CD7 antigen (p41) antibody
- CD7 antigen antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab1160 被引用在 2 文献中.
- Porwit-MacDonald A et al. BIOMED-1 concerted action report: flow cytometric characterization of CD7+ cell subsets in normal bone marrow as a basis for the diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL). Leukemia 14:816-25 (2000). PubMed: 10803512
- Thurlow PJ et al. A monoclonal antibody detecting a new human T cell antigen, HuLy-m2. Transplantation 38:143-7 (1984). PubMed: 6205484