重组Anti-PDX1抗体[EPR22002] (ab219207)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22002] to PDX1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PDX1抗体[EPR22002]
参阅全部 PDX1 一抗 -
描述
兔单克隆抗体[EPR22002] to PDX1 -
宿主
Rabbit -
特异性
Human and rat species are recommended based on IHC result. This antibody is unsuitable to be used in western blot on human and rat samples.
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经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse Beta-TC-6 whole cell lysate. IHC-P: Mouse, rat and human pancreas tissues. ICC/IF: Beta-TC-6 cells. IP: Beta-TC-6 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22002 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab219207于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 31 kDa).
Human and rat species are recommended based on IHC result. This antibody is unsuitable to be used in western blot on human and rat samples. |
|
IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Perform heat-mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. |
|
ICC/IF |
1/1000.
|
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IP |
1/30.
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说明 |
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WB
1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 31 kDa). Human and rat species are recommended based on IHC result. This antibody is unsuitable to be used in western blot on human and rat samples. |
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. |
ICC/IF
1/1000. |
IP
1/30. |
靶标
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功能
Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. -
组织特异性
Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). -
疾病相关
Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
序列相似性
Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
Contains 1 homeobox DNA-binding domain. -
结构域
The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y). -
翻译后修饰
Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3651 Human
- Entrez Gene: 18609 Mouse
- Entrez Gene: 29535 Rat
- Omim: 600733 Human
- SwissProt: P52945 Human
- SwissProt: P52946 Mouse
- SwissProt: P52947 Rat
- Unigene: 32938 Human
see all -
别名
- Glucose sensitive factor antibody
- Glucose-sensitive factor antibody
- GSF antibody
see all
图片
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All lanes : Anti-PDX1 antibody at 1/1000 dilution
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 31 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lane 1: 60 seconds
Lane 2: 7 seconds
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Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining in mouse pancreatic islet (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.
Performed on a Leica Biosystems BOND® RX instrument.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-PDX1 antibody [EPR22002] (ab219207) at 1/1000 dilution
Lane 1 : Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 92 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 12488243)
Negative control: NIH/3T3 (PMID: 25271154)
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling PDX1 with ab219207 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining in Beta-TC-6 cells.
Negative control: NIH/3T3 (PMID: 25271154).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining in rat pancreatic islet (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.
Performed on a Leica Biosystems BOND® RX instrument.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining on human pancreatic islet and pancreatic acini (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.
Performed on a Leica Biosystems BOND® RX instrument.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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PFX1 was immunoprecipitated from 0.35 mg of Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate with ab219207 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219207 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Beta-TC-6 whole cell lysate 10 µg (Input).
Lane 2: ab219207 IP in Beta-TC-6 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219207 in Beta-TC-6 whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 12488243).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab219207 被引用在 2 文献中.
- Li Y Gypenoside A attenuates dysfunction of pancreatic β cells by activating PDX1 signal transduction via the inhibition of miR-150-3p both in vivo and in vitro. J Biochem Mol Toxicol 36:e23004 (2022). PubMed: 35191145
- Zhang X et al. Amino acids-Rab1A-mTORC1 signaling controls whole-body glucose homeostasis. Cell Rep 34:108830 (2021). PubMed: 33730578