Anti-PDPK1抗体(ab2495)

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Western blot - Anti-PDPK1 antibody (ab2495)

    Key features and details

    • Rabbit polyclonal to PDPK1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-PDPK1抗体
      参阅全部 PDPK1 一抗

    • 描述

      兔多克隆抗体to PDPK1

    • 宿主

      Rabbit

    • 特异性

      This antibody is primarily directed against 60,000 MW human PDPK1 and is useful in determining its presence in various assays. It predominantly recognizes a 60 kDa band on Western blots.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Peptide sequence:

      RKIQEVWRQRYQS

      (amino acids 301-312).
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab2495 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB
  • 应用说明
    Western Blot: use at 1/750 to detect a 60 kD band.
    (previously used at this dilution to detect PDK 1 in lysates derived from Sf9 insect cells infected with virus expressing myc-tagged human PDK 1.
    Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation.

    • 靶标

    • 功能

      Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.

    • 组织特异性

      Appears to be expressed ubiquitously.

    • 序列相似性

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.

    • 翻译后修饰

      Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.

    • 细胞定位

      Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • 3 phosphoinositide dependent protein kinase 1 antibody
        • 3-phosphoinositide-dependent protein kinase 1 antibody
        • hPDK 1 antibody
        • hPDK1 antibody
        • MGC20087 antibody
        • MGC35290 antibody
        • OTTHUMP00000159109 antibody
        • OTTHUMP00000159110 antibody
        • OTTHUMP00000174525 antibody
        • PDK1 antibody
        • Pdpk1 antibody
        • PDPK1_HUMAN antibody
        • PDPK2 antibody
        • PDPK2P antibody
        • PkB kinase antibody
        • PkB kinase like gene 1 antibody
        • PkB like 1 antibody
        • PRO0461 antibody
        • Protein kinase antibody
        see all

      图片

      • Western blot - Anti-PDPK1 antibody (ab2495)
        Western blot - Anti-PDPK1 antibody (ab2495)

        Immunoblot of Sf9 insect cell lysate. Affinity purified anti-Human PDK 1 detects 60 kDa human PDK-1 in lysates from myc-tagged human PDK-1 virus infected Sf9 cells.  A 1/750 dilution is suggested followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection.  Other detection systems will yield similar results.

        Immunoblot of Sf9 insect cell lysate. Affinity purified anti-Human PDPK1 detects 60 kDa human PDPK1 in lysates from myc-tagged human PDPK1 virus infected Sf9 cells. A 1/750 dilution is suggested followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection. Other detection systems will yield similar results.

      文献 (0)

      发表研究结果有使用 ab2495？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab2495 尚未被引用在任何文献中。

      客户评价及客户问答

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      Question

      Customer would like to know what are positive controls for ab2808 and ab2495.

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      Answer

      Thank you for your phone call. I have enquired with the originators of these two antibodies and for ab2808, rat skeletal muscle homogenate should be used as a positive control and for ab2495, the originator used myc-tagged human PDK-1 virus infected Sf9 cells to test the antibody. This is all the information that we have at this time, if you have any more questions please contact us again.

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