Anti-PDPK1抗体(ab2495)
Key features and details
- Rabbit polyclonal to PDPK1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PDPK1抗体
参阅全部 PDPK1 一抗 -
描述
兔多克隆抗体to PDPK1 -
宿主
Rabbit -
特异性
This antibody is primarily directed against 60,000 MW human PDPK1 and is useful in determining its presence in various assays. It predominantly recognizes a 60 kDa band on Western blots. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
应用说明
Western Blot: use at 1/750 to detect a 60 kD band.
(previously used at this dilution to detect PDK 1 in lysates derived from Sf9 insect cells infected with virus expressing myc-tagged human PDK 1.
Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation.
(previously used at this dilution to detect PDK 1 in lysates derived from Sf9 insect cells infected with virus expressing myc-tagged human PDK 1.
Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation.
靶标
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功能
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. -
组织特异性
Appears to be expressed ubiquitously. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. -
细胞定位
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5170 Human
- Omim: 605213 Human
- SwissProt: O15530 Human
- Unigene: 459691 Human
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别名
- 3 phosphoinositide dependent protein kinase 1 antibody
- 3-phosphoinositide-dependent protein kinase 1 antibody
- hPDK 1 antibody
see all
图片
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Western blot - Anti-PDPK1 antibody (ab2495)Immunoblot of Sf9 insect cell lysate. Affinity purified anti-Human PDK 1 detects 60 kDa human PDK-1 in lysates from myc-tagged human PDK-1 virus infected Sf9 cells. A 1/750 dilution is suggested followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection. Other detection systems will yield similar results.
Immunoblot of Sf9 insect cell lysate. Affinity purified anti-Human PDPK1 detects 60 kDa human PDPK1 in lysates from myc-tagged human PDPK1 virus infected Sf9 cells. A 1/750 dilution is suggested followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection. Other detection systems will yield similar results.
数据表及文件
文献 (0)
ab2495 尚未被引用在任何文献中。
