概述

  • 产品名称
  • 描述
    兔多克隆抗体to PDI
  • 宿主
    Rabbit
  • 特异性
    PDI runs at 61.2 kD on samples from human placenta. In samples from rat liver, it runs at 59 kD, but 3 other bands may appear due to protein degradation - these run at 58.9 kD, 55.2 kD and 53.8 kD.
  • 经测试应用
    适用于: ICC, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Cow, Human, African green monkey, Chinese hamster
  • 免疫原

    Full length protein.

性能

应用

Our Abpromise guarantee covers the use of ab3672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 60 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/100.
ICC/IF 1/600. PubMed: 19059915

靶标

  • 功能
    Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
  • 组织特异性
    Highly expressed in pancreas (at protein level).
  • 序列相似性
    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • 翻译后修饰
    The disulfide-linked homodimer exhibits an enhanced chaperone activity.
    Glycosylated.
  • 细胞定位
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Pancreas specific protein disulfide isomerase antibody
    • Pancreas-specific protein disulfide isomerase antibody
    • Pancreatic protein disulfide isomerase antibody
    • PDA2 antibody
    • PDI antibody
    • PDIA2 antibody
    • PDIA2_HUMAN antibody
    • PDIp antibody
    • PDIR antibody
    • Protein disulfide isomerase A2 antibody
    • Protein disulfide isomerase antibody
    • Protein disulfide isomerase associated 2 antibody
    • Protein disulfide isomerase family A member 2 antibody
    • Protein disulfide isomerase pancreatic antibody
    • Protein disulfide-isomerase A2 antibody
    • Rho GDP dissociation inhibitor gamma antibody
    see all

图片

  • ICC/IF image of ab3672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3672, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

  • Anti-PDI antibody (ab3672) at 1/1000 dilution + PC12 whole cell lysate at 20 µg

    Secondary
    HRP conjugated swine anti-rabbit antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 60 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 36 kDa, 48 kDa, 58 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes

    See Abreview

  • ab3672 at 1/400 staining mouse kidney tissue sections by IHC-P. The tissue was formladehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer. The tissue was incubated with the primary antibody for 30 minutes at 22°C and then an HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Chinese Hamster Ovary cells stained for PDI using ab3672 at 1/200 dilution ICC/IF. Cy3®-labeled secondary antibody.

  • ab3672 staining mouse cortical oligodendrocytes from vibratome sections by IHC-Fr.  Sections were fixed in Somogyi (PFA + glutaraldehyde + picric acid) or PLP (Periodate-lysine-PFA) and permeabilized in TX-100, prior to blocking with 2.5% serum for 2 hours at 25°C.  The primary antibody was diluted 1/1000 and incubated with the sample for 18 hours at 25°C.  A TRITC conjugated pig anti-rabbit IgG antibody, diluted 1/1000, was used as the secondary.

    See Abreview

  • ab3672 staining PDI - ER in human HaCaT Keratinocyte by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.25% triton X-100 buffer and blocked in 2.5% BSA plus 1% goat serum for 1 hour at 4°C. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 24°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.

    See Abreview

文献

This product has been referenced in:
  • Wang H  et al. N-glycosylation in the protease domain of trypsin-like serine proteases mediates calnexin-assisted protein folding. Elife 7:N/A (2018). Read more (PubMed: 29889025) »
  • Charpak-Amikam Y  et al. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20. Sci Rep 7:8661 (2017). Read more (PubMed: 28819195) »
See all 18 Publications for this product

客户评价及客户问答

1-10 of 15 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Specification
HEK293
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

提交于 Dec 03 2012

Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese Hamster Cell (ovary)
Specification
ovary
Fixative
Methanol
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

提交于 Oct 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HaCaT keratinocyte)
Specification
HaCaT keratinocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% triton X-100
Blocking step
2.5% BSA plus 1% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Jun 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Cortical oligodendrocytes in vibratome sections)
Specification
Cortical oligodendrocytes in vibratome sections
Fixative
Somogy's. PFA + picric acid PLP also, PFA + lysine + periodate
Permeabilization
Yes - TX100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Dr. Guillermo Estivill-Torrus

Verified customer

提交于 Oct 31 2007

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa S3)
Total protein in input
500 µg
Specification
HeLa S3
Immuno-precipitation step
Protein A

Abcam user community

Verified customer

提交于 Oct 30 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cell line)
Loading amount
30 µg
Specification
CHO cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount
30 µg
Specification
3T3 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (adrenal tumour (phaeochromocytoma))
Specification
adrenal tumour (phaeochromocytoma)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Sep 10 2007

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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