Anti-PDHX抗体(ab232798)
Key features and details
- Rabbit polyclonal to PDHX
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-PDHX抗体
参阅全部 PDHX 一抗 -
描述
兔多克隆抗体to PDHX -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, Pig -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Human PDHX aa 200-500. (Expressed in E.coli).
Database link: O00330 -
阳性对照
- WB: Recombinant human PDHX protein; A431 and HeLa cell lysates; Pig heart lysate. ICC/IF: HeLa cells. IHC-P: Human stomach cancer, breast cancer, prostate cancer, prostate, stomach and bile duct cancer tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232798于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 54 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 54 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
靶标
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功能
Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. -
疾病相关
Defects in PDHX are the cause of pyruvate dehydrogenase E3-binding protein deficiency (PDHXD) [MIM:245349]. -
序列相似性
Belongs to the 2-oxoacid dehydrogenase family.
Contains 1 lipoyl-binding domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 8050 Human
- Omim: 608769 Human
- SwissProt: O00330 Human
- Unigene: 502315 Human
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别名
- Dihydrolipoamide dehydrogenase binding protein of pyruvate dehydrogenase complex antibody
- Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex antibody
- DLDBP antibody
see all
图片
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Anti-PDHX antibody (ab232798) at 3 µg/ml + Recombinant human PDHX protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 54 kDa -
All lanes : Anti-PDHX antibody (ab232798) at 3 µg/ml
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Pig heart lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 54 kDa -
Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human bile duct cancer tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PDHX (green) using ab232798 at 20 μg/ml in ICC/IF. FITC-labeled secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab232798 尚未被引用在任何文献中。