重组HRP Anti-PDGFR alpha + PDGFR beta抗体[Y92] (ab195515)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y92] to PDGFR alpha + PDGFR beta
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-PDGFR alpha + PDGFR beta抗体[Y92] -
描述
HRP兔单克隆抗体[Y92] to PDGFR alpha + PDGFR beta -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: FFPE human normal kidney tissue sections. WB: N-GST tagged Human PDGF Receptor beta (aa557 to 1106) recombinant protein, N-GST tagged Human PDGF Receptor alpha (aa550 to 1089) recombinant protein.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y92 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab196376)
- Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206872)
- Alexa Fluor® 405 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206873)
- Anti-PDGFR alpha + PDGFR beta antibody [Y92] - Low endotoxin, Azide free (ab215978)
- APC Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (ab314942)
- Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (ab32570)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab195515于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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细胞定位
PDGFR alpha: Membrane. PDGFR beta: Membrane. -
数据库链接
- Entrez Gene: 5156 Human
- Entrez Gene: 5159 Human
- Entrez Gene: 18595 Mouse
- Entrez Gene: 18596 Mouse
- Entrez Gene: 24629 Rat
- Entrez Gene: 25267 Rat
- Omim: 173410 Human
- Omim: 173490 Human
see all
图片
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IHC image of PDGFR alpha + beta staining in a section of formalin-fixed paraffin-embedded human normal kidney*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab195515 at 1µg/ml. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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