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Cardiovascular Angiogenesis Growth Factors Other

Anti-PDGFR alpha (phospho Y754)抗体(ab5460)

  • Datasheet
Reviews (7)Q&A (1)References (8)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)

Key features and details

  • Rabbit polyclonal to PDGFR alpha (phospho Y754)
  • Suitable for: IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-PDGFR alpha (phospho Y754)抗体
    参阅全部 PDGFR alpha 一抗
  • 描述

    兔多克隆抗体to PDGFR alpha (phospho Y754)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Human PDGFR alpha.
    (Peptide available as ab5695)

  • 阳性对照

    • IHC: Mouse colo, human brain tissue.
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at tyrosine 754.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • PDGF
    • Cancer
    • Growth factors
    • PDGF
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human PDGFR alpha protein (ab84797)

应用

Our Abpromise guarantee covers the use of ab5460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/20.

靶标

  • 功能

    Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • 组织特异性

    Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • 疾病相关

    Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P16234 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5156 Human
    • Entrez Gene: 18595 Mouse
    • Entrez Gene: 25267 Rat
    • Omim: 173490 Human
    • SwissProt: P16234 Human
    • SwissProt: P26618 Mouse
    • SwissProt: P20786 Rat
    • Unigene: 74615 Human
    • Unigene: 221403 Mouse
    • Unigene: 55127 Rat
    see all
  • 别名

    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF Receptor alpha antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)

    Paraffin embedded mouse colon tissue stained for PDGFR alpha (phospho Y754) using ab5460 (right) at 1/20 dilution compared to a negative control section without primary antibody (left), followed by HRP-conjugated secondary antibody in immunohistochemical analysis.

    Heat mediated antigen retrieval was performed using 10mM sodium citrate (pH 6.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)

    Paraffin embedded human brain tissue stained for PDGFR alpha (phospho Y754) usinf ab5460 (right) at 1/20 dilution compared to a negative control section without primary antibody (left), followed by HRP-conjugated secondary antibody in immunohistochemical analysis.

    Heat mediated antigen retrieval was performed using 10mM sodium citrate (pH 6.0).

实验方案

  • Peptide Competition Experiment
  • Western blotting protocol

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (8)

    发表研究结果有使用 ab5460?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5460 被引用在 8 文献中.

    • Saini H  et al. The role of tumor-stroma interactions on desmoplasia and tumorigenicity within a microengineered 3D platform. Biomaterials 247:119975 (2020). PubMed: 32278213
    • Wang J  et al. Activation PDGFR-a/AKT Mediated Signaling Pathways in Oral Squamous Cell Carcinoma by Mesenchymal Stem/Stromal Cells Promotes Anti-apoptosis and Decreased Sensitivity to Cisplatin. Front Oncol 10:552 (2020). PubMed: 32411595
    • Yang H  et al. Predictive and prognostic value of phosphorylated c-KIT and PDGFRA in advanced non-small cell lung cancer harboring ALK fusion. Oncol Lett 17:3071-3076 (2019). PubMed: 30867736
    • Ishihara S  et al. Suppressive Effects of Aspirin for Postthoracotomy Pleural Adhesion in Rats. Int J Med Sci 16:593-601 (2019). PubMed: 31171911
    • Kishi M  et al. Blockade of platelet-derived growth factor receptor-ß, not receptor-a ameliorates bleomycin-induced pulmonary fibrosis in mice. PLoS One 13:e0209786 (2018). PubMed: 30596712
    • Kobayashi K  et al. Involvement of PARK2-Mediated Mitophagy in Idiopathic Pulmonary Fibrosis Pathogenesis. J Immunol 197:504-16 (2016). PubMed: 27279371
    • Johnson TV  et al. Identification of retinal ganglion cell neuroprotection conferred by platelet-derived growth factor through analysis of the mesenchymal stem cell secretome. Brain N/A:N/A (2013). PubMed: 24176979
    • Sabha N  et al. Investigation of the In Vitro Therapeutic Efficacy of Nilotinib in Immortalized Human NF2-Null Vestibular Schwannoma Cells. PLoS One 7:e39412 (2012). WB ; Human . PubMed: 22745749

    客户评价及客户问答

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    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha (phospho Y754) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (GI tract)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    GI tract
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 02 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha (phospho Y754) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    colon
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 29 2017

    Question

    Really weak signal in WB

    Read More

    Abcam community

    Verified customer

    Asked on Jul 16 2012

    Answer

    Thank you for calling Abcam earlier today.

    I am sorry about the issues you are having with ab5460 in western blot. As we discussed I am sending you a new vial of the antibody to see if that helps resolves the situation. Your new order number is *.

    If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    回复于 Jul 16 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PDGFR alpha (phospho Y754) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human glioblastoma cell line)
    Permeabilization
    Yes - 0,1% Triton X-100
    Specification
    human glioblastoma cell line
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 17 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PDGFR alpha (phospho Y754) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human glioblastoma cell lines)
    Permeabilization
    Yes - Tritonx100 0.1% in PBS
    Specification
    human glioblastoma cell lines
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 29 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha (phospho Y754) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (glioblastoma multiforme brain section)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrit acid pH 6.1
    Permeabilization
    Yes - 0,3 % H202
    Specification
    glioblastoma multiforme brain section
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 15 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha (phospho Y754) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrit acid pH 6.1
    Permeabilization
    Yes - 0,3 % H202
    Specification
    brain
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 15 2010

    Western blot abreview for Anti-PDGFR alpha (phospho Y754) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (7,5)
    Loading amount
    150 µg
    Treatment
    PDGF 40ng/ml 5min
    Specification
    Mouse Fibroblasts
    Blocking step
    PhosphoBlocker as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 29 2010

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