Anti-PDGFR alpha (phospho Y754)抗体(ab5460)
Key features and details
- Rabbit polyclonal to PDGFR alpha (phospho Y754)
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PDGFR alpha (phospho Y754)抗体
参阅全部 PDGFR alpha 一抗 -
描述
兔多克隆抗体to PDGFR alpha (phospho Y754) -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human PDGFR alpha.
(Peptide available asab5695) -
阳性对照
- IHC: Mouse colo, human brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at tyrosine 754. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (4) |
1/20.
|
| 说明 |
|---|
|
IHC-P
1/20. |
靶标
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功能
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. -
组织特异性
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue. -
疾病相关
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5156 Human
- Entrez Gene: 18595 Mouse
- Entrez Gene: 25267 Rat
- Omim: 173490 Human
- SwissProt: P16234 Human
- SwissProt: P26618 Mouse
- SwissProt: P20786 Rat
- Unigene: 74615 Human
see all -
别名
- Alpha-type platelet-derived growth factor receptor antibody
- CD140 antigen-like family member A antibody
- CD140a antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)
Paraffin embedded mouse colon tissue stained for PDGFR alpha (phospho Y754) using ab5460 (right) at 1/20 dilution compared to a negative control section without primary antibody (left), followed by HRP-conjugated secondary antibody in immunohistochemical analysis.
Heat mediated antigen retrieval was performed using 10mM sodium citrate (pH 6.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)Paraffin embedded human brain tissue stained for PDGFR alpha (phospho Y754) usinf ab5460 (right) at 1/20 dilution compared to a negative control section without primary antibody (left), followed by HRP-conjugated secondary antibody in immunohistochemical analysis.
Heat mediated antigen retrieval was performed using 10mM sodium citrate (pH 6.0).
实验方案
数据表及文件
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Datasheet download
文献 (10)
ab5460 被引用在 10 文献中.
- Saini H et al. The role of tumor-stroma interactions on desmoplasia and tumorigenicity within a microengineered 3D platform. Biomaterials 247:119975 (2020). PubMed: 32278213
- Wang J et al. Activation PDGFR-a/AKT Mediated Signaling Pathways in Oral Squamous Cell Carcinoma by Mesenchymal Stem/Stromal Cells Promotes Anti-apoptosis and Decreased Sensitivity to Cisplatin. Front Oncol 10:552 (2020). PubMed: 32411595
- Altunel E et al. Development of a precision medicine pipeline to identify personalized treatments for colorectal cancer. BMC Cancer 20:592 (2020). PubMed: 32580713
- Detweiler ND et al. Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension. J Appl Physiol (1985) 127:393-407 (2019). PubMed: 31169471
- Yang H et al. Predictive and prognostic value of phosphorylated c-KIT and PDGFRA in advanced non-small cell lung cancer harboring ALK fusion. Oncol Lett 17:3071-3076 (2019). PubMed: 30867736
- Ishihara S et al. Suppressive Effects of Aspirin for Postthoracotomy Pleural Adhesion in Rats. Int J Med Sci 16:593-601 (2019). PubMed: 31171911
- Kishi M et al. Blockade of platelet-derived growth factor receptor-ß, not receptor-a ameliorates bleomycin-induced pulmonary fibrosis in mice. PLoS One 13:e0209786 (2018). PubMed: 30596712
- Kobayashi K et al. Involvement of PARK2-Mediated Mitophagy in Idiopathic Pulmonary Fibrosis Pathogenesis. J Immunol 197:504-16 (2016). PubMed: 27279371
- Johnson TV et al. Identification of retinal ganglion cell neuroprotection conferred by platelet-derived growth factor through analysis of the mesenchymal stem cell secretome. Brain N/A:N/A (2013). PubMed: 24176979
- Sabha N et al. Investigation of the In Vitro Therapeutic Efficacy of Nilotinib in Immortalized Human NF2-Null Vestibular Schwannoma Cells. PLoS One 7:e39412 (2012). WB ; Human . PubMed: 22745749
