Name: Anti-PDGFR alpha antibody [EPR5480]
Brand: RabMAb
SKU: ab134123
Rating: 3 (3 reviews)

Product price, shipping and contact information

Currently unavailable

抱歉，我们目前无法显示此内容。

请联系我们以完成下单，或者之后再尝试访问。

正在加载规格和价格。。。

Abpromise

产品质量保证，客户专家支持，

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5480] to PDGFR alpha
Suitable for: Flow Cyt (Intra), ELISA, WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human, Recombinant fragment

Carrier Free

产品名称

Anti-PDGFR alpha抗体[EPR5480]
参阅全部 PDGFR alpha 一抗
描述

兔单克隆抗体[EPR5480] to PDGFR alpha
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), ELISA, WB, IHC-Pmore details
种属反应性

与反应: Mouse, Rat, Human, Recombinant fragment
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- IHC-P: Human colonic carcinoma, human colon tissue. WB: SH SY5Y, C2C12, Human skeletal muscle, Human spleen, and Human fetal brain lysates. Flow Cyt (intra): SH-SY5Y, L6 cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR5480
同种型

IgG
研究领域
- Neuroscience
- Development

Alternative Versions
- Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human PDGFRA knockout SH-SY5Y cell line (ab275335)
KO cell lysates
- Human PDGFRA knockout SH-SY5Y cell lysate (ab275522)
Recombinant Protein
- Recombinant human PDGFR alpha protein (ab84797)
Related Products
- Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

The Abpromise guarantee

Abpromise™承诺保证使用ab134123于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/20.
ELISA		Use at an assay dependent concentration.
WB		1/1000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 122 kDa).
IHC-P	(3)	1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

说明
Flow Cyt (Intra) 1/20.
ELISA Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 122 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

功能

Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
组织特异性

Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
疾病相关

Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
序列相似性

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
细胞定位

Membrane.
Target information above from: UniProt accession P16234 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 5156 Human
- Entrez Gene: 18595 Mouse
- Entrez Gene: 25267 Rat
- Omim: 173490 Human
- SwissProt: P16234 Human
- SwissProt: P26618 Mouse
- SwissProt: P20786 Rat
- Unigene: 74615 Human
- Unigene: 221403 Mouse
- Unigene: 55127 Rat
see all
别名
- Alpha-type platelet-derived growth factor receptor antibody
- CD140 antigen-like family member A antibody
- CD140a antibody
- CD140a antigen antibody
- MGC74795 antibody
- PDGF alpha chain antibody
- PDGF Receptor alpha antibody
- PDGF-R-alpha antibody
- PDGFR 2 antibody
- PDGFR alpha antibody
- PDGFR2 antibody
- PDGFRA antibody
- PDGFRA/BCR fusion antibody
- PGFRA_HUMAN antibody
- Platelet derived growth factor receptor 2 antibody
- Platelet derived growth factor receptor alpha antibody
- Platelet derived growth factor receptor alpha polypeptide antibody
- Platelet derived growth factor receptor antibody
- Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
- RHEPDGFRA antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling PDGFR alpha with ab134123 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells in human colonic carcinoma. The section was incubated with ab134123 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

Lane 1 : Wild-type SH-SY5Y cell lysate
Lane 2 : PDGFRA knockout SH-SY5Y cell lysate

Lysates/proteins at 40 µg per lane.

Performed under reducing conditions.

Predicted band size: 122 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?

Lanes 1 - 2: Merged signal (red and green). Green - ab134123 observed at 180 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab134123 was shown to react with PDGFR alpha in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRA knockout cell line ab275335 (knockout cell lysate ab275522). Wild-type SH-SY5Y and PDGFRA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab134123 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

Intracellular Flow Cytometry analysis of L6 (Rat skeletal muscle myoblast) cells labelling PDGFR alpha with ab134123 at 1/20 dilution (14.65 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : Human spleen lysate
Lane 3 : Human fetal brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 122 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PDGFR alpha with ab134123 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells in human colon (PMID: 23429582). The section was incubated with ab134123 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling PDGFR alpha with ab134123 at 1/20 dilution (14.65µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
ELISA - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

ELISA analysis of Human PDGFR alpha recombinant protein at 250 ng/mL with ab134123. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Lane 2 : C2C12 (mouse myoblast cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 122 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Anti-PDGFR alpha antibody [EPR5480] (ab134123)

Western blot protocols

Click here to view the general protocols

Datasheet download

Download

发表研究结果有使用 ab134123？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab134123 被引用在 4 文献中.

Du Y et al. The Epithelial to Mesenchymal Transition Related Gene Calumenin Is an Adverse Prognostic Factor of Bladder Cancer Correlated With Tumor Microenvironment Remodeling, Gene Mutation, and Ferroptosis. Front Oncol 11:683951 (2021). PubMed: 34150647
Landry NM et al. An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture. Sci Rep 9:12889 (2019). PubMed: 31501457
Wang Y et al. Necrostatin-1 ameliorates the pathogenesis of experimental autoimmune encephalomyelitis by suppressing apoptosis and necroptosis of oligodendrocyte precursor cells. Exp Ther Med 18:4113-4119 (2019). PubMed: 31611942
Schmid FM et al. IFT20 modulates ciliary PDGFRa signaling by regulating the stability of Cbl E3 ubiquitin ligases. J Cell Biol 217:151-161 (2018). PubMed: 29237719

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer pH 9.0

Permeabilization

Specification

skin

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 19 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6

Permeabilization

Specification

skin

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 11 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Embryonic lung)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Embryonic lung

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Jul 13 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

Datasheet download

文献 (4)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha antibody [EPR5480]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha antibody [EPR5480]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGF Receptor alpha antibody [EPR5480]