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使用敲除细胞株进行验证重组RabMAb

重组Anti-PDGFR alpha抗体[EPR5480] (ab134123)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
  • Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
  • Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
  • Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
  • Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
  • ELISA - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
  • Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
  • Anti-PDGFR alpha antibody [EPR5480] (ab134123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5480] to PDGFR alpha
  • Suitable for: Flow Cyt (Intra), ELISA, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

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概述

  • 产品名称

    Anti-PDGFR alpha抗体[EPR5480]
    参阅全部 PDGFR alpha 一抗
  • 描述

    兔单克隆抗体[EPR5480] to PDGFR alpha
  • 宿主

    Rabbit
  • 经测试应用

    适用于: Flow Cyt (Intra), ELISA, WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Recombinant fragment
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human colonic carcinoma, human colon tissue. WB: SH SY5Y, C2C12, Human skeletal muscle, Human spleen, and Human fetal brain lysates. Flow Cyt (intra): SH-SY5Y, L6 cells.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR5480
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • PDGF
    • Cancer
    • Growth factors
    • PDGF
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Neuroscience
    • Development

相关产品

  • Alternative Versions

    • Anti-PDGFR alpha antibody [EPR5480] - BSA and Azide free (ab248689)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PDGFRA knockout SH-SY5Y cell line (ab275335)
  • KO cell lysates

    • Human PDGFRA knockout SH-SY5Y cell lysate (ab275522)
  • Recombinant Protein

    • Recombinant human PDGFR alpha protein (ab84797)
  • Related Products

    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab134123于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/20.
ELISA
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 122 kDa).
IHC-P (3)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

说明
Flow Cyt (Intra)
1/20.
ELISA
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 122 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

靶标

  • 功能

    Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • 组织特异性

    Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • 疾病相关

    Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P16234 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5156 Human
    • Entrez Gene: 18595 Mouse
    • Entrez Gene: 25267 Rat
    • Omim: 173490 Human
    • SwissProt: P16234 Human
    • SwissProt: P26618 Mouse
    • SwissProt: P20786 Rat
    • Unigene: 74615 Human
    • Unigene: 221403 Mouse
    • Unigene: 55127 Rat
    see all
  • 别名

    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF Receptor alpha antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

    Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling PDGFR alpha with ab134123 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells in human colonic carcinoma. The section was incubated with ab134123 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

    Lane 1 : Wild-type SH-SY5Y cell lysate
    Lane 2 : PDGFRA knockout SH-SY5Y cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 122 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab134123 observed at 180 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab134123 was shown to react with PDGFR alpha in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRA knockout cell line ab275335 (knockout cell lysate ab275522). Wild-type SH-SY5Y and PDGFRA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab134123 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

    Intracellular Flow Cytometry analysis of L6 (Rat skeletal muscle myoblast) cells labelling PDGFR alpha with ab134123 at 1/20 dilution (14.65 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

     

  • Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human spleen lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 122 kDa
    Observed band size: 190 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PDGFR alpha with ab134123 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells in human colon (PMID: 23429582). The section was incubated with ab134123 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

    Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling PDGFR alpha with ab134123 at 1/20 dilution (14.65µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue). 

     

     

  • ELISA - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    ELISA - Anti-PDGFR alpha antibody [EPR5480] (ab134123)

    ELISA analysis of Human PDGFR alpha recombinant protein at 250 ng/mL with ab134123. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
    Lane 2 : C2C12 (mouse myoblast cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 122 kDa
    Observed band size: 190 kDa why is the actual band size different from the predicted?

  • Anti-PDGFR alpha antibody [EPR5480] (ab134123)
    Anti-PDGFR alpha antibody [EPR5480] (ab134123)

实验方案

  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (4)

发表研究结果有使用 ab134123?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab134123 被引用在 4 文献中.

  • Du Y  et al. The Epithelial to Mesenchymal Transition Related Gene Calumenin Is an Adverse Prognostic Factor of Bladder Cancer Correlated With Tumor Microenvironment Remodeling, Gene Mutation, and Ferroptosis. Front Oncol 11:683951 (2021). PubMed: 34150647
  • Landry NM  et al. An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture. Sci Rep 9:12889 (2019). PubMed: 31501457
  • Wang Y  et al. Necrostatin-1 ameliorates the pathogenesis of experimental autoimmune encephalomyelitis by suppressing apoptosis and necroptosis of oligodendrocyte precursor cells. Exp Ther Med 18:4113-4119 (2019). PubMed: 31611942
  • Schmid FM  et al. IFT20 modulates ciliary PDGFRa signaling by regulating the stability of Cbl E3 ubiquitin ligases. J Cell Biol 217:151-161 (2018). PubMed: 29237719

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha antibody [EPR5480]

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Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer pH 9.0
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 19 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGFR alpha antibody [EPR5480]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 11 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGF Receptor alpha antibody [EPR5480]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Embryonic lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Embryonic lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Jul 13 2016

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