Anti-PDGFR alpha + Beta (phospho Y572 + Y574)抗体(ab5443)
Key features and details
- Rabbit polyclonal to PDGFR alpha + Beta (phospho Y572 + Y574)
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PDGFR alpha + Beta (phospho Y572 + Y574)抗体 -
描述
兔多克隆抗体to PDGFR alpha + Beta (phospho Y572 + Y574) -
宿主
Rabbit -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Xenopus laevis -
免疫原
Synthetic peptide corresponding to Human PDGFR alpha + Beta aa 550-650 (phospho Y572 + Y574). This corresponds to Tyrosine 579 and 581 of Human PDGFR beta. The sequence is conserved in PDGFR alpha mouse, rat and frog.
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阳性对照
- WB: NIH/3T3 cells +/- PDGF; ICC: MCF7 cells treated with PDGF .
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha / beta. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at Tyrosines 572 and 574. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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ICC |
1/100 - 1/500.
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WB |
1/1000. Detects a band of approximately 170 - 185 kDa.
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说明 |
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ICC
1/100 - 1/500. |
WB
1/1000. Detects a band of approximately 170 - 185 kDa. |
靶标
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相关性
Platelet derived growth factor receptor (PDGFR) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo- or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF-AA, -BB or -AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation. PDGFR alpha tyrosines 572 and 574 (579 and 581 in PDGFR beta) are autophosphorylated in the activated receptor, and bind and activate Src family kinases. -
细胞定位
Membrane; single pass type I membrane protein. -
数据库链接
- Entrez Gene: 5156 Human
- Entrez Gene: 5159 Human
- Entrez Gene: 18595 Mouse
- Entrez Gene: 18596 Mouse
- Entrez Gene: 24629 Rat
- Entrez Gene: 25267 Rat
- Omim: 173410 Human
- Omim: 173490 Human
see all -
别名
- CD140A antibody
- CD140a antigen antibody
- CD140b antibody
see all
图片
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Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50
µ g/mL ab5443 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5443 blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF -
Immunocytochemistry analysis of 70% confluent log phase MCF7 cells treated with PDGF (50 ng/mL for 10 min) labeling PDGFR alpha + Beta (phospho Y572 + Y574) with ab5443. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ab5443 at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin. Panel d is a merged image showing cytoplasmic and membrane localization. Panel e shows untreated cells. Panel f shows no primary antibody control. The images were captured at 20X magnification.
实验方案
数据表及文件
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Datasheet download
文献 (4)
ab5443 被引用在 4 文献中.
- Mahadik K et al. c-Abl-TWIST1 Epigenetically Dysregulate Inflammatory Responses during Mycobacterial Infection by Co-Regulating Bone Morphogenesis Protein and miR27a. Front Immunol 9:85 (2018). PubMed: 29449840
- Ip CKM et al. Neomorphic PDGFRA extracellular domain driver mutations are resistant to PDGFRA targeted therapies. Nat Commun 9:4583 (2018). PubMed: 30389923
- Csak T et al. MicroRNA-155 Deficiency Attenuates Liver Steatosis and Fibrosis without Reducing Inflammation in a Mouse Model of Steatohepatitis. PLoS One 10:e0129251 (2015). WB ; Mouse . PubMed: 26042593
- Paugh BS et al. Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. Cancer Res 73:6219-29 (2013). Human . PubMed: 23970477