Anti-PDE6D抗体(ab96825)
Key features and details
- Rabbit polyclonal to PDE6D
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PDE6D抗体
参阅全部 PDE6D 一抗 -
描述
兔多克隆抗体to PDE6D -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Zebrafish -
免疫原
Recombinant fragment corresponding to Human PDE6D aa 1-120.
Database link: NP_002592 -
阳性对照
- WB: MOLT4 whole cell lysate; mouse eye lysate. IHC-P: SAS xenograft tissue. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96825于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/5000. Predicted molecular weight: 17 kDa.
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说明 |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/5000. Predicted molecular weight: 17 kDa. |
靶标
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功能
Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. -
组织特异性
Retina. -
序列相似性
Belongs to the PDE6D/unc-119 family. - Information by UniProt
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数据库链接
- Entrez Gene: 281976 Cow
- Entrez Gene: 403781 Dog
- Entrez Gene: 5147 Human
- Entrez Gene: 18582 Mouse
- Entrez Gene: 363272 Rat
- Omim: 602676 Human
- SwissProt: Q95142 Cow
- SwissProt: Q9XT54 Dog
see all -
别名
- 5''-cyclic phosphodiesterase subunit delta antibody
- GMP PDE delta antibody
- GMP-PDE delta antibody
see all
图片
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Paraffin-embedded SAS xenograft tissue labeling PDE6D with ab96825 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EDTA-based (pH 8.0), 15 minutes.
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Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PDE6D with ab96825 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Anti-PDE6D antibody (ab96825) at 1/5000 dilution + Mouse eye lysate at 50 µg
Secondary
Anti-Rabbit IgG HRP
Predicted band size: 17 kDa -
Anti-PDE6D antibody (ab96825) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 17 kDa
12% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab96825 被引用在 1 文献中.
- Chen YH et al. EPHA2 feedback activation limits the response to PDEd inhibition in KRAS-dependent cancer cells. Acta Pharmacol Sin N/A:N/A (2019). PubMed: 31316177