Anti-PDE6 beta/PDE6B抗体(ab5663)
Key features and details
- Rabbit polyclonal to PDE6 beta/PDE6B
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
概述
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产品名称
Anti-PDE6 beta/PDE6B抗体 -
描述
兔多克隆抗体to PDE6 beta/PDE6B -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IHC-Frmore details -
种属反应性
与反应: Mouse, Cow, Human
预测可用于: Dog
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免疫原
Synthetic peptide corresponding to Mouse PDE6 beta/PDE6B aa 20-36.
Sequence:HQYFG(K/R)KLSPENVAGAC
(Peptide available asab5890) -
常规说明
This product was previously labelled as PDE6 beta
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha’ (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha’/alpha’ homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa. | |
| IHC-Fr | Use at an assay dependent concentration. |
靶标
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功能
This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme. -
疾病相关
Defects in PDE6B are the cause of retinitis pigmentosa type 40 (RP40) [MIM:613801]. RP40 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Defects in PDE6B are a cause of congenital stationary night blindness autosomal dominant type 2 (CSNBAD2) [MIM:163500]; also known as congenital stationary night blindness Rambusch type. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 281974 Cow
- Entrez Gene: 399653 Dog
- Entrez Gene: 5158 Human
- Entrez Gene: 18587 Mouse
- Omim: 180072 Human
- SwissProt: P23439 Cow
- SwissProt: P33726 Dog
- SwissProt: P35913 Human
see all -
别名
- 5''-cyclic phosphodiesterase subunit beta antibody
- Congenital stationary night blindness 3 autosomal dominant antibody
- CSNB 3 antibody
see all
图片
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Western blot - Anti-PDE6 beta/PDE6B antibody (ab5663)Western blot of PDE6 beta/PDE6B using ab5663.
Lane 1 demostrates no detection on extracts of RD mice.
Lane 2 shows a single band at ~90 kDa on Balb/C extracts. -
Immunohistochemistry (Frozen sections) - Anti-PDE6 beta/PDE6B antibody (ab5663)Immunofluorescences on frozen secions of mouse retina outer segments after staining with ab5663.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 beta/PDE6B antibody (ab5663)IHC image of ab5663 staining in mouse normal eye formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5663, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
文献 (8)
ab5663 被引用在 8 文献中.
- Yan W et al. A Natural Occurring Mouse Model with Adgrv1 Mutation of Usher Syndrome 2C and Characterization of its Recombinant Inbred Strains. Cell Physiol Biochem 47:1883-1897 (2018). PubMed: 29961073
- Sanges D et al. Reprogramming Müller glia via in vivo cell fusion regenerates murine photoreceptors. J Clin Invest 126:3104-3116 (2016). WB ; Mouse . PubMed: 27427986
- Emoto Y et al. Green tea extract suppresses N-methyl-N-nitrosourea-induced photoreceptor apoptosis in Sprague-Dawley rats. Graefes Arch Clin Exp Ophthalmol 252:1377-84 (2014). PubMed: 25012920
- Demontis GC et al. Functional and molecular characterization of rod-like cells from retinal stem cells derived from the adult ciliary epithelium. PLoS One 7:e33338 (2012). ICC/IF ; Mouse . PubMed: 22432014
- Grossman GH et al. Immunocytochemical evidence of Tulp1-dependent outer segment protein transport pathways in photoreceptor cells. Exp Eye Res 93:658-68 (2011). PubMed: 21867699
- Goto M et al. Phosphodiesterase inhibitors block the acceleration of skin permeability barrier repair by red light. Exp Dermatol 20:568-71 (2011). IHC-Fr ; Mouse . PubMed: 21410772
- Giordano F et al. Fibroblast growth factor and epidermal growth factor differently affect differentiation of murine retinal stem cells in vitro. Mol Vis 13:1842-50 (2007). ICC/IF ; Mouse . PubMed: 17960120
- Olshevskaya EV et al. The Y99C mutation in guanylyl cyclase-activating protein 1 increases intracellular Ca2+ and causes photoreceptor degeneration in transgenic mice. J Neurosci 24:6078-85 (2004). WB ; Mouse . PubMed: 15240799
