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Signal Transduction Second Messenger Nucleotide Messenger cAMP

Anti-PDE6 beta/PDE6B抗体(ab5663)

  • Datasheet
Reviews (1)Q&A (2)References (8)

Product price, shipping and contact information

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Western blot - Anti-PDE6 beta/PDE6B antibody (ab5663)
  • Immunohistochemistry (Frozen sections) - Anti-PDE6 beta/PDE6B antibody (ab5663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 beta/PDE6B antibody (ab5663)

Key features and details

  • Rabbit polyclonal to PDE6 beta/PDE6B
  • Suitable for: IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-PDE6 beta/PDE6B抗体
  • 描述

    兔多克隆抗体to PDE6 beta/PDE6B
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, WB, IHC-Frmore details
  • 种属反应性

    与反应: Mouse, Cow, Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide corresponding to Mouse PDE6 beta/PDE6B aa 20-36.
    Sequence:

    HQYFG(K/R)KLSPENVAGAC


    (Peptide available as ab5890)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

     This product was previously labelled as PDE6 beta

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha’ (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha’/alpha’ homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP
    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cGMP
    • Neuroscience
    • Sensory System
    • Visual system

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human PDE6 beta/PDE6B protein (ab125593)

应用

Our Abpromise guarantee covers the use of ab5663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能

    This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.
  • 疾病相关

    Defects in PDE6B are the cause of retinitis pigmentosa type 40 (RP40) [MIM:613801]. RP40 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in PDE6B are a cause of congenital stationary night blindness autosomal dominant type 2 (CSNBAD2) [MIM:163500]; also known as congenital stationary night blindness Rambusch type. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision.
  • 序列相似性

    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P35913 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 281974 Cow
    • Entrez Gene: 399653 Dog
    • Entrez Gene: 5158 Human
    • Entrez Gene: 18587 Mouse
    • Omim: 180072 Human
    • SwissProt: P23439 Cow
    • SwissProt: P33726 Dog
    • SwissProt: P35913 Human
    • SwissProt: P23440 Mouse
    • Unigene: 623810 Human
    • Unigene: 654544 Human
    • Unigene: 1372 Mouse
    see all
  • 别名

    • 5''-cyclic phosphodiesterase subunit beta antibody
    • Congenital stationary night blindness 3 autosomal dominant antibody
    • CSNB 3 antibody
    • CSNB3 antibody
    • CSNBAD2 antibody
    • GMP PDE beta antibody
    • GMP-PDE beta antibody
    • PDE 6 beta antibody
    • PDE 6B antibody
    • PDE6B antibody
    • PDE6B_HUMAN antibody
    • PDEB antibody
    • Phosphodiesterase 6B antibody
    • Phosphodiesterase 6B cGMP specific rod beta antibody
    • Rd 1 antibody
    • Rd1 antibody
    • Rod cGMP phosphodiesterase beta subunit antibody
    • Rod cGMP specific 3' 5' cyclic phosphodiesterase beta subunit antibody
    • Rod cGMP-specific 3'' antibody
    • RP40 antibody
    see all

图片

  • Western blot - Anti-PDE6 beta/PDE6B antibody (ab5663)
    Western blot - Anti-PDE6 beta/PDE6B antibody (ab5663)

    Western blot of PDE6 beta/PDE6B using ab5663.
    Lane 1 demostrates no detection on extracts of RD mice.
    Lane 2 shows a single band at ~90 kDa on Balb/C extracts.

  • Immunohistochemistry (Frozen sections) - Anti-PDE6 beta/PDE6B antibody (ab5663)
    Immunohistochemistry (Frozen sections) - Anti-PDE6 beta/PDE6B antibody (ab5663)

    Immunofluorescences on frozen secions of mouse retina outer segments after staining with ab5663.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 beta/PDE6B antibody (ab5663)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 beta/PDE6B antibody (ab5663)
    IHC image of ab5663 staining in mouse normal eye formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5663, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (8)

    发表研究结果有使用 ab5663?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5663 被引用在 8 文献中.

    • Yan W  et al. A Natural Occurring Mouse Model with Adgrv1 Mutation of Usher Syndrome 2C and Characterization of its Recombinant Inbred Strains. Cell Physiol Biochem 47:1883-1897 (2018). PubMed: 29961073
    • Sanges D  et al. Reprogramming Müller glia via in vivo cell fusion regenerates murine photoreceptors. J Clin Invest 126:3104-3116 (2016). WB ; Mouse . PubMed: 27427986
    • Emoto Y  et al. Green tea extract suppresses N-methyl-N-nitrosourea-induced photoreceptor apoptosis in Sprague-Dawley rats. Graefes Arch Clin Exp Ophthalmol 252:1377-84 (2014). PubMed: 25012920
    • Demontis GC  et al. Functional and molecular characterization of rod-like cells from retinal stem cells derived from the adult ciliary epithelium. PLoS One 7:e33338 (2012). ICC/IF ; Mouse . PubMed: 22432014
    • Grossman GH  et al. Immunocytochemical evidence of Tulp1-dependent outer segment protein transport pathways in photoreceptor cells. Exp Eye Res 93:658-68 (2011). PubMed: 21867699
    • Goto M  et al. Phosphodiesterase inhibitors block the acceleration of skin permeability barrier repair by red light. Exp Dermatol 20:568-71 (2011). IHC-Fr ; Mouse . PubMed: 21410772
    • Giordano F  et al. Fibroblast growth factor and epidermal growth factor differently affect differentiation of murine retinal stem cells in vitro. Mol Vis 13:1842-50 (2007). ICC/IF ; Mouse . PubMed: 17960120
    • Olshevskaya EV  et al. The Y99C mutation in guanylyl cyclase-activating protein 1 increases intracellular Ca2+ and causes photoreceptor degeneration in transgenic mice. J Neurosci 24:6078-85 (2004). WB ; Mouse . PubMed: 15240799

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Question

    Phone call requesting information as to whether the anti-PDE6 beta antibody (ab5663) can be used with rat tissue.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 06 2012

    Answer

    Thank you for contacting us and sorry for the delay in getting back to you.

    Unfortunately I can confirm that the have not tested the Anti-PDE6 beta antibody (ab5663) with Rat samples. The image which was on the datasheet was unfortunately mislabeled. This has now been corrected. Thank you for bringing this to our attention.

    Although ab5663 has not been tested with rat samples this does not mean that it would not work.Due to the high sequence homology of the immunogen used to raise the antibody with therat protein we would expect the antibody to cross react, however as we have not tested it we would not be able to guarantee this.

    However, if you would like to purchase ab5663 and use it with yourrat samples, we can offer you a testing discount. This offer means that if you purchase ab5663 as normal, test the antibody with your rat samples and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab5663 off any product). More information on this offer can be found here:

    www.abcam.com/collaborationdiscount

    Please not that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab5663.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    回复于 Jul 06 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-PDE6 beta/PDE6B antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skin, Retina)
    Permeabilization
    Yes - 0.4% TritonX-100
    Specification
    Skin, Retina
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 11 2011

    Question

    My question is on the immunohistochemistry application using anti-pde 6b antibody (Ab5663). There is a picture of a mouse retinal section stained with this antibody in the Abcam information site on Ab5663. What antibody dilution was used to obtain that retinal picture? I have already purchased the antibody, it gave me excellent results on Western blot, however I am not sure about the immunohistochemistry on mouse sections from retina. I only have a limited nr. of samples and I can not titer it at the moment. I would like to know of a reasonable dilution (I am aware there is no way of getting it perfectly right) to start with. Thank you in advance for your help.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 23 2004

    Answer

    Sorry for the delayed response. I have been unable to confirm the precise dilutions used in this image but would recommend starting at 1:100 and move to higher dilutions from there.

    Read More

    Abcam Scientific Support

    回复于 Sep 09 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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