Anti-PDE6 alpha抗体(ab5659)
Key features and details
- Rabbit polyclonal to PDE6 alpha
- Suitable for: IHC-P, WB
- Reacts with: Sheep, Human
- Isotype: IgG
概述
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产品名称
Anti-PDE6 alpha抗体
参阅全部 PDE6 alpha 一抗 -
描述
兔多克隆抗体to PDE6 alpha -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Sheep, Human
预测可用于: Dog
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免疫原
Synthetic peptide corresponding to Human PDE6 alpha aa 1-16.
Sequence:MGEVTAEEVEKFLDSN
(Peptide available asab5862) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa. representing PDE6 alpha from sheep retinal extract. A higher molecular weight band is sometimes observed using sheep retinal extracts. See Guo et al, Kerov et al for details of WB procedure.
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| 说明 |
|---|
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa. representing PDE6 alpha from sheep retinal extract. A higher molecular weight band is sometimes observed using sheep retinal extracts. See Guo et al, Kerov et al for details of WB procedure. |
靶标
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功能
This protein participates in processes of transmission and amplification of the visual signal. -
疾病相关
Defects in PDE6A are the cause of retinitis pigmentosa type 43 (RP43) [MIM:613810]. RP43 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 403620 Dog
- Entrez Gene: 5145 Human
- Omim: 180071 Human
- SwissProt: Q28263 Dog
- SwissProt: P16499 Human
- Unigene: 151710 Human
- Unigene: 567314 Human
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别名
- 5''-cyclic phosphodiesterase subunit alpha antibody
- CGPR A antibody
- GMP PDE alpha antibody
see all
图片
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Western blot - Anti-PDE6 alpha antibody (ab5659)
Western blot of PDE6 alpha on sheep retinal extracts using ab5659.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 alpha antibody (ab5659)IHC image of ab5659 staining in human renal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5659, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab5659 被引用在 12 文献中.
- Wong BH et al. Mfsd2a Is a Transporter for the Essential ?-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development. J Biol Chem 291:10501-14 (2016). PubMed: 27008858
- Sarfare S et al. Overexpression of rod photoreceptor glutamic acid rich protein 2 (GARP2) increases gain and slows recovery in mouse retina. Cell Commun Signal 12:67 (2014). WB ; Mouse . PubMed: 25323447
- Zhong X et al. Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs. Nat Commun 5:4047 (2014). PubMed: 24915161
- Wert KJ et al. Gene therapy provides long-term visual function in a pre-clinical model of retinitis pigmentosa. Hum Mol Genet 22:558-67 (2013). WB ; Mouse . PubMed: 23108158
- Wert KJ et al. Mid-stage intervention achieves similar efficacy as conventional early-stage treatment using gene therapy in a pre-clinical model of retinitis pigmentosa. Hum Mol Genet N/A:N/A (2013). WB ; Human . PubMed: 24101599