Anti-PDE3B抗体(ab99290)
Key features and details
- Rabbit polyclonal to PDE3B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PDE3B抗体
参阅全部 PDE3B 一抗 -
描述
兔多克隆抗体to PDE3B -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Chicken, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to a region between residue 1062 and 1112 of human PDE3B. (NP_000913.2)
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阳性对照
- HeLa whole cell lysate (ab150035)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. -
组织特异性
Abundant in adipose tissues. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5140 Human
- Omim: 602047 Human
- SwissProt: Q13370 Human
- Unigene: 445711 Human
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别名
- 5''-cyclic phosphodiesterase B antibody
- Adipocyte cAMP phosphodiesterase cGMP inhibited antibody
- AI847709 antibody
see all
图片
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All lanes : Anti-PDE3B antibody (ab99290) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 124 kDa -
Lane 1: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody recognising an upstream epitope of PDE3B.
Lane 2: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab99290 at 3 µg/mg lysate for IP.
Lane 3 : Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab99290 at 1 µg/ml. Developed using the ECL technique with an exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab99290 被引用在 2 文献中.
- Svart M et al. Oral 3-hydroxybutyrate ingestion decreases endogenous glucose production, lipolysis, and hormone-sensitive lipase phosphorylation in adipose tissue in men: a human randomized, controlled, crossover trial. Diabet Med 38:e14385 (2021). PubMed: 32794582
- Ramshanker N et al. Effects of short-term prednisolone treatment on indices of lipolysis and lipase signaling in abdominal adipose tissue in healthy humans. Metabolism 99:1-10 (2019). PubMed: 31260678