Anti-PD1抗体[J43] (ab95789)
Key features and details
- Armenian hamster monoclonal [J43] to PD1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-PD1抗体[J43]
参阅全部 PD1 一抗 -
描述
亚美尼亚仓鼠单克隆抗体[J43] to PD1 -
宿主
Armenian hamster -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse PD1. Syrian Hamster kidney cell line BKH transfected with Mouse PD1 cDNA.
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阳性对照
- 3-day ConA activated BALB/c Mouse splenocytes.
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常规说明
It is reported that monoclonal antibody J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
It is reported that monoclonal antibody J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2. -
克隆
单克隆 -
克隆编号
J43 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95789于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 0.5µg for 105-8 cells.
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use 0.5µg for 105-8 cells. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Possible cell death inducer, in association with other factors. -
疾病相关
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
发展阶段
Induced at programmed cell death. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 18566 Mouse
- SwissProt: Q02242 Mouse
- Unigene: 5024 Mouse
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别名
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
图片
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Flow cytometry staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c Mouse splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control (open histogram) or 0.25 µg of ab95789 (filled histogram) followed by Anti-Armenian Hamster IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.
数据表及文件
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Datasheet download
文献 (3)
ab95789 被引用在 3 文献中.
- Sahyon HA & Al-Harbi SA Chemoprotective role of an extract of the heart of the Phoenix dactylifera tree on adriamycin-induced cardiotoxicity and nephrotoxicity by regulating apoptosis, oxidative stress and PD-1 suppression. Food Chem Toxicol 135:111045 (2020). PubMed: 31830546
- Ansari MJ et al. The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice. J Exp Med 198:63-9 (2003). PubMed: 12847137
- Salama AD et al. Critical role of the programmed death-1 (PD-1) pathway in regulation of experimental autoimmune encephalomyelitis. J Exp Med 198:71-8 (2003). PubMed: 12847138