重组Anti-PD-L1抗体[BLR020E] (ab243877)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR020E] to PD-L1
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Human
概述
-
产品名称
Anti-PD-L1抗体[BLR020E]
参阅全部 PD-L1 一抗 -
描述
兔单克隆抗体[BLR020E] to PD-L1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-P, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human PD-L1 aa 250-290 (C terminal). The exact sequence is proprietary. NP_054862.1 and Gene ID 29126.
Database link: Q9NZQ7 -
常规说明
This product is sold under License from Bethyl Laboratories, Inc.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
-
纯化说明
Recombinant antibody was purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
BLR020E -
同种型
IgG -
研究领域
相关产品
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab243877于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000.
|
|
ICC/IF |
1/100 - 1/500.
|
|
IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
IP |
Use at an assay dependent concentration.
Use 20µl/mg lysate. |
说明 |
---|
WB
1/1000. |
ICC/IF
1/100 - 1/500. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. Use 20µl/mg lysate. |
靶标
-
功能
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
组织特异性
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane and Endomembrane system. - Information by UniProt
-
数据库链接
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
-
别名
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
图片
-
PD-L1 was immunoprecipitated from HeLa RKO cell lysate (1.0 mg per IP reaction, 20% loaded) with ab243877 at 20 µl/mg lysate. Western blot was performed on the immunoprecipitate using ab243877 at 1/1000 dilution.
Lane 1: rabbit anti-PD-L1 recombinant monoclonal antibody IP in RKO whole cell lysate.
Lane 2: ab243877 IP in RKO whole cell lysate.
Lane 3: ab243877 IP in RKO whole cell lysate.
Lane 4: Control IgG in RKO whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD-L1 with ab243877 and secondary HRP-conjugated goat anti-rabbit IgG.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Sup-M2 cells labelling PD-L1 with ab243877 and secondary HRP-conjugated goat anti-rabbit IgG.
-
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PD-L1 using ab243877 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. Counterstain was DAPI.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PD-L1 with ab243877 and HRP-conjugated goat anti-rabbit secondary. CD8 alpha is in red, and CD3E is in green. Counterstain is DAPI (blue)
-
PDL-1 was immunoprecipitated from SUP-M2 whole cell lysate with ab243877. Western blot was performed on the immunoprecipitate using ab243877 at 1/1000 dilution.
Lane 1: ab243877 IP in SUP-M2 whole cell lysate.
Lane 2: Contol IgG in SUP-M2 whole cell lysate.
Detection: Chemiluminescence.
-
All lanes : Anti-PD-L1 antibody [BLR020E] (ab243877) at 1/1000 dilution
Lane 1 : SUP-M2 whole cell lysate
Lane 2 : HDLM-2 whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : Ramos whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : PD-L2 whole cell lysate
Lane 7 : PD-L1 whole cell lysate
Lane 8 : HEK-293 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG antibodyDetection: chemiluminescence.
-
Formaldehyde-fixed HDML-2 cells labeling PDL-1 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
-
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A DyLight®-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab243877 被引用在 1 文献中.
- Bahnassy AA et al. The prognostic role of PD-1, PD-L1, ALK, and ROS1 proteins expression in non-small cell lung carcinoma patients from Egypt. J Egypt Natl Canc Inst 34:23 (2022). PubMed: 35644823