Anti-PD-L1抗体[ABM4E54] (ab210931)
Key features and details
- Mouse monoclonal [ABM4E54] to PD-L1
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-PD-L1抗体[ABM4E54]
参阅全部 PD-L1 一抗 -
描述
小鼠单克隆抗体[ABM4E54] to PD-L1 -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human PD-L1 aa 1-250.
Database link: Q9NZQ7-1 -
阳性对照
- Recombinant protein fragment of Human PD-L1; Human Hodkin's lymphoma tissue. Programmed death-ligand 1 (PD-L1) expression in various tumor types - http://www.immunotherapyofcancer.org/content/1/S1/P53
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
ABM4E54 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab210931于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 33 kDa.
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Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 33 kDa. |
Flow Cyt
Use a concentration of 1 - 2 µg/ml. |
靶标
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功能
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
组织特异性
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane and Endomembrane system. - Information by UniProt
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数据库链接
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
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别名
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
图片
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Immunohistochemical analysis of formalin-fixed paraffin-embedded Human Hodkin's lymphoma tissue, labeling PD-L1 with ab210931at 5 µg/mL.
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Anti-PD-L1 antibody [ABM4E54] (ab210931) at 0.5 µg/ml + Recombinant protein fragment of Human PD-L1
Predicted band size: 33 kDa -
Cell Surface flow cytometry analysis of PD-L1 in PHA treated human PBMC using 1 μg of ab210931. Green represents isotype control; pink represents ab210931 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
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All lanes : Anti-PD-L1 antibody [ABM4E54] (ab210931) at 2 µg/ml
Lane 1 : A549 lysate
Lane 2 : MCF-7 lysate
Lane 3 : 293 lysate
Lane 4 : HCT-116 lysate
Lane 5 : Saos2 lysate
Lane 6 : HeLa lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDaExposure time: 1 minute in film and 30 seconds in imager.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab210931 被引用在 21 文献中.
- Park TI et al. Routine culture and study of adult human brain cells from neurosurgical specimens. Nat Protoc 17:190-221 (2022). PubMed: 35022619
- Li J et al. Gestational trophoblastic neoplasia with extrauterine metastasis but lacked uterine primary lesions: a single center experience and literature review. BMC Cancer 22:509 (2022). PubMed: 35524210
- Zhang Q et al. USP35 is a Potential Immunosuppressive Factor in Skin Cutaneous Melanoma. J Inflamm Res 15:3065-3082 (2022). PubMed: 35637872
- He HX et al. VISTA and PD-L1 synergistically predict poor prognosis in patients with extranodal natural killer/T-cell lymphoma. Oncoimmunology 10:1907059 (2021). PubMed: 33889438
- Ahn JH et al. A Novel Anti-PD-L1 Antibody Exhibits Antitumor Effects on Multiple Myeloma in Murine Models via Antibody-Dependent Cellular Cytotoxicity. Biomol Ther (Seoul) 29:166-174 (2021). PubMed: 33139584