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RabMAb

Anti-PD-L1抗体[28-8] (ab205921)

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概述

  • 产品名称
    Anti-PD-L1抗体[28-8]
    参阅全部 PD-L1 一抗
  • 描述
    兔单克隆抗体[28-8] to PD-L1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human PD-L1 (extracellular). The immunogen contains the specific extracellular domain of huPD-L1 (Phe19-Thr239). See reference for more info - www.ncbi.nlm.nih.gov/pmc/articles/PMC4561627/
    Database link: Q9NZQ7

  • 阳性对照
    • Tissue: Human tonsil, head and neck squamous cell carcinoma and placenta tissues; L2987 cell line. Cell Lines: Positives: B-CPAP- high, ES-2- medium, HCC70 - low CHO-PDL1 U-87 MG For additional information, please refer to here: Programmed death-ligand 1 (PD-L1) expression in various tumor types - http://www.immunotherapyofcancer.org/content/1/S1/P53
  • 常规说明

    Information on positive controls (Chinese version found here)

    Tissue:
    Tonsil- with hyperreactive changes
    Recommended to screen hyper-reactive tonsils to find those with highest expression of PD-L1 in crypt epithelium, macrophages homing the germinal centers and interfollicular mononuclear leukocytes.

    Tumor tissues- prescreened for positive tumor and inflammatory infiltrates
    PD-L1 expression varies by tumor type so screening is recommended to find positive and negative tumor controls.

    The following publication is useful for suggesting suitable tumor types:
    http://www.immunotherapyofcancer.org/content/1/S1/P53

    Note: Look for specimens with high numbers of inflammatory macrophages and mononuclear leukocytes.

    Cell Lines: 
    Positive controls: B-CPAP- high, ES-2- medium, HCC70 – low,
    Negative controls: COLO205, see ab95356.

    Primary negative control, isotype control:  RabMAb negative control antibody, ab172730.

    Recombinant protein: Recombinant human PD-L1 protein, ab167713.

    Recommended protocols:

    For recommended Western Blotting (WB) protocol, Flow Cytometry (FC) protocol and Immunohistochemistry (IHC) protocol, please refer to the protocol section, here.

    IHC usage:
    For IHC usage on FFPE tissues, we recommend using PD-L1 IHC panel ab236676, which contains PD-L1 clone 28-8, Universal HIER antigen retrieval reagent (ab208572) and IHC detection kit HRP/DAB (ab209101).

    Western blot usage:
    For clone 28-8, it is recommended to use Odyssey system. This system has the advantages of a wider dynamic range and less background than chemiluminescence.

    Alternative versions available:

    Conjugated versions of the antibody available (Alexa Fluor® 488, Alexa Fluor® 647, PE, HRP) - see list.

    A low endotoxin, azide free version of the antibody is available: ab209889.

    Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit: ab214565.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab205921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Boil slides seven times for five minutes each in pH 6 citrate buffer.

For antigen buffer for FFPE tissue, it is recommended to use Universal HIER antigen retrieval reagent (ab208572).
WB Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 2 µg/ml.

靶标

  • 功能
    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • 组织特异性
    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • 序列相似性
    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位
    Cell membrane and Endomembrane system.
  • Information by UniProt
  • 数据库链接
    • 别名
      • B7 H antibody
      • B7 H1 antibody
      • B7 homolog 1 antibody
      • B7-H1 antibody
      • B7H antibody
      • B7H1 antibody
      • CD 274 antibody
      • CD274 antibody
      • CD274 antigen antibody
      • CD274 molecule antibody
      • MGC142294 antibody
      • MGC142296 antibody
      • OTTHUMP00000021029 antibody
      • PD L1 antibody
      • PD-L1 antibody
      • PD1L1_HUMAN antibody
      • PDCD1 ligand 1 antibody
      • PDCD1L1 antibody
      • PDCD1LG1 antibody
      • PDL 1 antibody
      • PDL1 antibody
      • Programmed cell death 1 ligand 1 antibody
      • Programmed death ligand 1 antibody
      • RGD1566211 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)Image from Nakamura S HL et al., PLoS One. 2017;12(10):e0186192 Fig 3.; doi: 10.1371/journal.pone.0186192.

      Representative images of PD-L1 expression in human lung adenocarcinoma and squamous cell carcinoma specimens.

      (A) <1.0%, (B) 1.0–4.9%, (C) 5.0–9.9%, (D) 10.0–49.9%, and (E) ≥50.0% PD-L1-positive cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemical analysis of Human Tonsil tissue with ab205921 at 2 µg/ml.
      PD-L1 positive expression of the crypt epithelium (large black arrow) and cells localized within the germinal centers (small black arrow)
      Note negative staining of the stroma (red arrow), additionally, stainings of follicles and some interfollicular cells

       

      For recommended Immunohistochemistry (IHC) protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Flow Cytometry - Anti-PD-L1 antibody [28-8] (ab205921)
      Flow Cytometry - Anti-PD-L1 antibody [28-8] (ab205921)

      Ab205921 specificity testing by Flow Cytometry (KO testing): Loss of detection on KO Cells
      Strong detection with anti-PD-L1 (ab205921, clone 28-8) TALEN constructs targeting exon4 of human PD-L1, transcript variant 1 (NM_014143.3) and complete knock out (K.O) confirmed by deep sequencing in clone L2-14. Cell surface staining is almost completely eliminated in the L2987 L2-14 K.O. cell line.

       

      For recommended Flow Cytometry (Flow Cyt) protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Western blot - Anti-PD-L1 antibody [28-8] (ab205921)
      Western blot - Anti-PD-L1 antibody [28-8] (ab205921)

      Primary ab Dilution 1:100 dilution, Secondary ab Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody, 1:20,000 dilution, Blocking and diluting buffer and concentration 5% NFDM/TBST, Lane 1: NCI-H1975 (Human non-small cell lung cancer epithelia), Observed MW 40-60 kDa, Exposure time 3 min.

      For recommended Western Blot (WB) protocol for endogenous PD-L1 expression, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Ab205921 specificity testing by Immunohistochemistry (KO testing): Loss of detection on KO Cells

      Strong IHC detection with anti-PD-L1 (ab205921, clone 28-8) is seen in human lung adenocarcinoma tumor cell line L2987. PDL1 gene was edited in L2987 cells using TALEN constructs targeting exon4 of human PD-L1, transcript variant 1 (NM_014143.3) and complete knock out (K.O) confirmed by deep sequencing in clone L2-14. IHC detection is completely eliminated in the L2987 L2-14 K.O. cell line.

       

      For recommended Immunohistochemistry (IHC) protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [28-8] (ab205921)This image is courtesy of an Abreview submitted by Dr. Dimitra Kalamida.

      Paraformaldehyde-fixed, Triton X-100 permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for PD-L1 (red) using ab205921 at 1/200 dilution in ICC/IF, followed by CF568 Donkey anti-rabbit IgG(H+L) secondary antibody at 1/500 dilution.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemical staining of PD-L1 in formalin fixed, paraffin embedded human non-squamous non-small cell lung cancer (NSQ-NSCLC) using ab205921 at a dilution of 1/400, incubated for an hour at room temperature. Heat mediated antigen retrieval was carried out in low pH buffer and the sample was blocked with peroxidase blocking buffer for 3 minutes.

      This image was courteously provided by Dr. Kai Schmitt from the Institute of Pathology, Saarbrücken-Rastpfuhl.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemical analysis of CHO PD-L1 cells with ab205921 at 2 µg/ml.
      High power view
      A) Rabbit IgG, 5 µg/mL. No staining
      B) Anti PD-L1, 2 µg/mL (ab205921 batches 1)
      C) Anti PD-L1, 2 µg/mL (ab205921 batches 3)
      D) Anti PD-L1, 2 µg/mL (ab205921 batches 4)
      E) Anti PD-L1, 2 µg/mL (ab205921 batches 5)
      F) Anti PD-L1, 2 µg/mL (ab205921 batches 6)
      G) Anti PD-L1, 2 µg/mL (ab205921 batches 7)

       

      All batches/lots (1,3,4,5,6,7) showed consistent results.


      Note strong, moderate, and weak (red, yellow, and white arrows respectively) plasma membrane staining of CHO PD-L1 transfected cells

       

      For recommended Immunohistochemistry (IHC) protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemical analysis of CHO Parental cells with ab205921 at 2 µg/ml.
      High power view
      A) Rabbit IgG, 5 µg/mL. No staining
      B) Anti PD-L1, 2 µg/mL (ab205921 batches 1)
      C) Anti PD-L1, 2 µg/mL (ab205921 batches 3)
      D) Anti PD-L1, 2 µg/mL (ab205921 batches 4)
      E) Anti PD-L1, 2 µg/mL (ab205921 batches 5)
      F) Anti PD-L1, 2 µg/mL (ab205921 batches 6)
      G) Anti PD-L1, 2 µg/mL (ab205921 batches 7)

       

      All batches/lots (1,3,4,5,6,7) showed consistent results.

       

      Note absence of PD-L1 expression in CHO parental cells.

       

      For recommended Immunohistochemistry (IHC) protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemical analysis of Human Lung NSCLC with ab205921 at 2 µg/ml.
      High power view
      A) Rabbit IgG, 5 µg/mL. No staining
      B) Anti PD-L1, 2 µg/mL (ab205921 batches 1)
      C) Anti PD-L1, 2 µg/mL (ab205921 batches 3)
      D) Anti PD-L1, 2 µg/mL (ab205921 batches 4)
      E) Anti PD-L1, 2 µg/mL (ab205921 batches 5)
      F) Anti PD-L1, 2 µg/mL (ab205921 batches 6)

       

      All batches/lots (1,3,4,5,6) showed consistent results.


      Note linear and complete or partial (arrows) PD-L1 staining of tumor cells. Tumor associated immune cells localized over the tumor margin exhibit positive plasma membrane staining (small arrows).

       

      For recommended Immunohistochemistry (IHC) protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling PD-L1 with ab205921. Staining can be seen in tumor associated macrophages and tumor cells.

      For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
      For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling PD-L1 with ab205921 on Ventana Ultra. Tumor cells and immune cells show PD-L1 positive plasma membrane staining.

      For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
      For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling PD-L1 with ab205921. Tumor cells and immuno cells localized within the stroma show PD-LA plasma membrane staining.

      For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
      For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling PD-L1 with ab205921. Tumor cells show weak and partial postive PD-L1 expresseion in the plasma membrane. PD-L1 positive tumor associated immunoe cells are also stained. 

      For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
      For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling PD-L1 with ab205921 at 2 µg/ml. Counterstained with Hematoxylin.

      For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
      For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human head and neck squamous cell carcinoma tissue labeling PD-L1 with ab205921 at 2 µg/ml. Counterstained with Hematoxylin.

      For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
      For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded PD-L1 negative Non-small cell lung carcinoma (NSCLC) tissue with ab205921 at 2 µg/ml. Counterstained with Hematoxylin.

      For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
      For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)This image is courtesy of an Abreview submitted by Mr. Rudolf Jung.

      Paraformaldehyde-fixed, paraffin-embedded human placenta tissue stained for PD-L1 using ab2205921 at 1/100 dilution in immunohistochemical analysis.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (ab205921)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded L2987 (Human lung adenocarcinoma cell line with endogenous PD-L1 expression) cells labeling PD-L1 with ab205921 at 2 µg/ml. Counterstained with Hematoxylin.

      For antigen retrival buffer, Universal HIER antigen retrieval reagent (ab208572) was used.
      For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

    • Western blot - Anti-PD-L1 antibody [28-8] (ab205921)
      Western blot - Anti-PD-L1 antibody [28-8] (ab205921)

      (A and B) Western blots of recombinant PD-L1 protein (Lane 1), cell lysates of CHO-PD-L1 (Lane 3), CHO (Lane 4), ES-2 (Lane 5) and Colo205 (Lane 6) cell lines. In B, anti-PD-L1 (ab205921, clone 28-8) was pre-incubated with purified recombinant PDL1 protein overnight at 4°C.

      Blank/no sample (Lane2). Lane 2 is blank on purpose.

      For recommended Western Blot (WB) protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    文献

    This product has been referenced in:
    • Le Flahec G  et al. Mismatch repair-deficient colorectal cancer: a model of immunogenic and immune cell-rich tumor despite nonsignificant programmed cell death ligand-1 expression in tumor cells. Hum Pathol 72:135-143 (2018). IHC-P ; Human . Read more (PubMed: 29208565) »
    • Sato K  et al. Correlation between immune-related adverse events and efficacy in non-small cell lung cancer treated with nivolumab. Lung Cancer 115:71-74 (2018). Read more (PubMed: 29290265) »
    See all 80 Publications for this product

    发表研究结果有使用 ab205921？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    1-9 of 9 Abreviews or Q&A

    Question


    Please advise what is the recommended negative control tissue for Anti-PD-L1 antibody [28-8] #ab205921 for IHC applications.


    Read More
    Answer

    Thank you for your inquiry.


    As negative control for PD-L1 I can recommend the COLO205 cell line (Ab95356).


    As far as tissue goes it is unfortunately not as straight forward as tumor Specimens- PD-L1 expression varies by tumor type so screening is recommended to find positive and negative tumor controls.


    We found a good negative control in a non-small cell lung cancer samples. But please be aware that many tumor specimens have some inflammatory macrophages and tumor associated immune cells which we would expect to be positive. This is why we recommend screening of different samples.


    Endothelial cells can serve as negative internal controls within a positive (tumor-) tissue.


    I hope you find this information helpful and wish you good luck for your research. Update: For negative control - COLO205 cell line, please see Ab95356

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PD-L1 antibody [28-8]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U87MG glioblastoma)
    Permeabilization
    Yes - 0.1% v/v Triton X-100 pH 7.4, 5 min at RT
    Specification
    U87MG glioblastoma
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    提交于 Sep 12 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (lymph node)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    lymph node
    Blocking step
    ultratek kit, ScyTek Laboratories, Logan, Utah, USA as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Sara Belluco

    Verified customer

    提交于 Sep 12 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Colon/Tonsil)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: pH9 20 mins 100oC
    Specification
    Colon/Tonsil
    Blocking step
    No blocking step used for 30 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 May 30 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-PD-L1 antibody [28-8]

     Good
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Ovarian Tumor)
    Permeabilization
    No
    Specification
    Ovarian Tumor
    Blocking step
    Peroxidase Blocking Solution + UV block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 May 25 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (NSQ-NSCLC)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EnVision FLEX TRS, Low pH
    Permeabilization
    No
    Specification
    NSQ-NSCLC
    Blocking step
    EnV FLEX Peroxidase-Blocking Reagent as blocking agent for 3 minute(s) · Concentration: 100%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 May 11 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

     Inconclusive
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Tumor generated by a carcinogen (DMBA) or PANC02 m)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6
    Permeabilization
    No
    Specification
    Tumor generated by a carcinogen (DMBA) or PANC02 m
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 15 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Commercial Wash Buffer with Tween
    Specification
    Placenta
    Fixative
    Paraformaldehyde
    Read More

    Mr. Rudolf Jung

    Verified customer

    提交于 Feb 04 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
    Permeabilization
    No
    Specification
    Placenta
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

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    提交于 Dec 04 2015

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