Key features and details
- Rabbit polyclonal to PCSK2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
参阅全部 PCSK2 一抗
经测试应用适用于: ICC/IF, WBmore details
种属反应性与反应: Mouse, Human
预测可用于: Rat, Cow, Pig
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
纯度Immunogen affinity purified
Primary antibody说明The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.
Our Abpromise guarantee covers the use of ab3533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/20 - 1/200.|
|WB||Use a concentration of 2 µg/ml.
This antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract.
功能Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues.
序列相似性Belongs to the peptidase S8 family. Furin subfamily.
细胞定位Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
- Information by UniProt
- KEX2 like endoprotease 2 antibody
- KEX2-like endoprotease 2 antibody
- NEC 2 antibody
Anti-PCSK2 antibody (ab3533) at 1/500 dilution + Mouse cerebellum cell lysate at 25 µg
Observed band size: 65 kDa why is the actual band size different from the predicted?
Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
ab3533 被引用在 3 文献中.
- Wu S et al. Apatinib inhibits tumor growth and angiogenesis in PNET models. Endocr Connect N/A:N/A (2018). PubMed: 30557852
- Du W et al. HID-1 is required for homotypic fusion of immature secretory granules during maturation. Elife 5:N/A (2016). WB, IF ; Mouse . PubMed: 27751232
- Gagnon J et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). PubMed: 18706454