Anti-PCSK2抗体(ab3533)

Submit a review Submit a question References (3)
Western blot - Anti-PCSK2 antibody (ab3533)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody (ab3533)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody (ab3533)

Key features and details

  • Rabbit polyclonal to PCSK2
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-PCSK2抗体
    参阅全部 PCSK2 一抗

  • 描述

    兔多克隆抗体to PCSK2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Cow, Pig

  • 免疫原

    Synthetic peptide corresponding to Mouse PCSK2 aa 622-638.
    Sequence:

    ELDEAVERSLQSILRKN


    (Peptide available as ab5012)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • Primary antibody说明

    The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/20 - 1/200.
WB Use a concentration of 2 µg/ml.

This antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract.

靶标

图片

  • Western blot - Anti-PCSK2 antibody (ab3533)
    Western blot - Anti-PCSK2 antibody (ab3533)
    Anti-PCSK2 antibody (ab3533) at 1/500 dilution + Mouse cerebellum cell lysate at 25 µg

    Observed band size: 65 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody (ab3533)
    Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody (ab3533)

    Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody (ab3533)
    Immunocytochemistry/ Immunofluorescence - Anti-PCSK2 antibody (ab3533)

    Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

文献 (3)

发表研究结果有使用 ab3533？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3533 被引用在 3 文献中.

  • Wu S  et al. Apatinib inhibits tumor growth and angiogenesis in PNET models. Endocr Connect N/A:N/A (2018). PubMed: 30557852
  • Du W  et al. HID-1 is required for homotypic fusion of immature secretory granules during maturation. Elife 5:N/A (2016). WB, IF ; Mouse . PubMed: 27751232
  • Gagnon J  et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). PubMed: 18706454

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

There are currently no Customer reviews or Questions for ab3533.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com