Application
Western blot
Sample
Human Cell lysate - whole cell (human colon cancer cells)
Loading amount
40 µg
Specification
human colon cancer cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/2500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: bsa 5% TBS tween 0,1%
Secondary antibody
Name
Non-Abcam antibody was used: Goat DAKO anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ecl +
Exposure
1 second(s)
Bands
Specific: 35 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 22 2013