Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody [EPR3821]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No

Other product details

Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexafluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were MeOH-fixed, immunofluorescently labeled and counterstained with DAPI. Under these conditions, this antibody produces a strong, nuclear enriched staining pattern. Paraformaldehyde-fixed samples were also evaluated and failed to produce the expected pattern.

Dr. Kirk Mcmanus

Verified customer

提交于 May 31 2012

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