Anti-PCNA抗体(ab152112)
Key features and details
- Rabbit polyclonal to PCNA
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PCNA抗体
参阅全部 PCNA 一抗 -
描述
兔多克隆抗体to PCNA -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Pig -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-253 of PCNA (Uniprot: P12004).
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阳性对照
- Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF7, NT2D1, PC3, U87 MG, NIH 3T3, JC, BCL1, PC12, A549 and SiHa whole cell lysates; HeLa cells; Human KYSE170 xenograft and Human breast carcinoma tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab152112于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
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IHC-P | (2) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
靶标
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功能
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
序列相似性
Belongs to the PCNA family. -
翻译后修饰
Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
细胞定位
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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数据库链接
- Entrez Gene: 5111 Human
- Entrez Gene: 18538 Mouse
- Entrez Gene: 692192 Pig
- Entrez Gene: 25737 Rat
- Omim: 176740 Human
- SwissProt: P12004 Human
- SwissProt: P17918 Mouse
- SwissProt: P04961 Rat
see all -
别名
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
图片
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All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : HCT116 whole cell lysate at 15 µg
Lane 2 : HCT116 whole cell lysate at 10 µg
Lane 3 : HCT116 whole cell lysate at 5 µg
Lane 4 : HCT116 whole cell lysate at 1 µg
Predicted band size: 29 kDa
12% SDS PAGE -
PCBP2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts with 5 µg ab152112. Western blot was performed from the immunoprecipitate using ab152112.
Lane 1: PCPB2 protein only.
Lane 2: IgG instead of ab152112 in HeLa whole cell extract.
Lane 3: ab152112 IP in HeLa whole cell extract.
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Paraffin-embedded mouseliver tissue stained for PCNA using ab152112 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human KYSE170 xenograft tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat hind brain tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PCNA with ab152112 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PCNA with ab152112 at 1/100 dilution.
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Immunofluorescent analysis of methanol-fixed HeLa cells labeling PCNA with ab152112 at 1/500 dilution.
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Immunofluorescent analysis of HeLa (Human epithelial cells from cervix adenocarcinoma cell line) labeling PCNA with ab152112 at 1/500 dilution.
Counterstain is Hoechst 33342 (blue).
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All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : HeLa whole cell lyate
Lane 6 : HepG2 whole cell lysate
Lane 7 : H1299 whole cell lysate
Lane 8 : HCT116 whole cell lysate
Lane 9 : MCF7 whole cell lysate
Lane 10 : NT2D1 whole cell lysate
Lane 11 : PC3 whole cell lysate
Lane 12 : U87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
All lanes : Anti-PCNA antibody (ab152112) at 1/1000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Anti-PCNA antibody (ab152112) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE -
All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : NIH 3T3 whole cell lysate
Lane 3 : PC12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Anti-PCNA antibody (ab152112) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab152112 被引用在 25 文献中.
- Jin M et al. Circ_0011292 knockdown mitigates progression and drug resistance in PTX-resistant non-small-cell lung cancer cells by regulating miR-433-3p/CHEK1 axis. Thorac Cancer 13:1276-1288 (2022). PubMed: 35348291
- Xu B et al. Circ_0017274 acts on miR-637/CDX2 axis to facilitate cisplatin resistance in gastric cancer. Clin Exp Pharmacol Physiol 49:1105-1115 (2022). PubMed: 35748299
- Gustorff C et al. GABAB Receptor-Mediated Impairment of Intermediate Progenitor Maturation During Postnatal Hippocampal Neurogenesis of Newborn Rats. Front Cell Neurosci 15:651072 (2021). PubMed: 34421540
- Wang J et al. Knockdown of long non-coding RNA PVT1 induces apoptosis of fibroblast-like synoviocytes through modulating miR-543-dependent SCUBE2 in rheumatoid arthritis. J Orthop Surg Res 15:142 (2020). PubMed: 32293498
- Feng C et al. Tumor-Suppressive MicroRNA-216b Binds to TPX2, Activating the p53 Signaling in Human Cutaneous Squamous Cell Carcinoma. Mol Ther Nucleic Acids 20:186-195 (2020). PubMed: 32169806