重组Anti-PCK1/PEPC抗体[EPR6939] (ab125003)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6939] to PCK1/PEPC
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PCK1/PEPC抗体[EPR6939]
参阅全部 PCK1/PEPC 一抗 -
描述
兔单克隆抗体[EPR6939] to PCK1/PEPC -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human PCK1/PEPC aa 600-700 (C terminal). The exact sequence is proprietary.
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阳性对照
- Human fetal kidney and Human adipose tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6939 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125003于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
靶标
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功能
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. -
组织特异性
Major sites of expression are liver, kidney and adipocytes. -
通路
Carbohydrate biosynthesis; gluconeogenesis. -
疾病相关
Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. -
序列相似性
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. -
翻译后修饰
Acetylation is increased on addition of glucose and appears to regulate the protein stability. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5105 Human
- Omim: 614168 Human
- SwissProt: P35558 Human
- Unigene: 1872 Human
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别名
- cytosolic [GTP] antibody
- GTP antibody
- PCK1 antibody
see all
图片
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All lanes : Anti-PCK1/PEPC antibody [EPR6939] (ab125003) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human adipose tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125003 尚未被引用在任何文献中。