Anti-PCB抗体- C-terminal (ab229267)
Key features and details
- Rabbit polyclonal to PCB - C-terminal
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-PCB抗体- C-terminal
参阅全部 PCB 一抗 -
描述
兔多克隆抗体to PCB - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IF, IPmore details -
种属反应性
与反应: Rat, Human
预测可用于: Cow, Pig, Rhesus monkey -
免疫原
Recombinant fragment within Human PCB (C terminal). The exact sequence is proprietary.
Database link: P11498 -
阳性对照
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract. IHC-P: Rat duodenum tissue. ICC/IF: HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab229267于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100 - 1/1000.
|
|
WB |
1/500 - 1/3000. Predicted molecular weight: 130 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
|
IP |
1/100 - 1/857.
|
说明 |
---|
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 130 kDa. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/857. |
靶标
-
功能
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. -
通路
Carbohydrate biosynthesis; gluconeogenesis. -
疾病相关
Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia. -
序列相似性
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
-
数据库链接
- Entrez Gene: 338471 Cow
- Entrez Gene: 5091 Human
- Entrez Gene: 397630 Pig
- Entrez Gene: 25104 Rat
- Entrez Gene: 713103 Rhesus monkey
- Omim: 608786 Human
- SwissProt: Q29RK2 Cow
- SwissProt: P11498 Human
see all -
别名
- mitochondrial antibody
- OTTHUMP00000235155 antibody
- OTTHUMP00000235156 antibody
see all
图片
-
All lanes : Anti-PCB antibody - C-terminal (ab229267) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 130 kDa5% SDS-PAGE gel.
-
PCB was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5μg ab229267. Western blot was performed from the immunoprecipitate using ab229267. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HeLa whole cell extract.
Lane 2: Control IgG instead of ab229267 in HeLa whole cell extract.
Lane 3: ab229267 IP in HeLa whole cell extract.
-
2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PCB (green) using ab229267 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
-
Paraffin-embedded rat duodenum tissue stained for PCB using ab229267 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab229267 被引用在 5 文献中.
- Wang J et al. NDRG2 inhibits pyruvate carboxylase-mediated anaplerosis and combines with glutamine blockade to inhibit the proliferation of glioma cells. Am J Cancer Res 12:3729-3744 (2022). PubMed: 36119843
- James J et al. Single Mutation in the NFU1 Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells. Arterioscler Thromb Vasc Biol 41:734-754 (2021). PubMed: 33297749
- Zhou Q et al. Effect of Gegen Qinlian Decoction on Hepatic Gluconeogenesis in ZDF Rats with Type 2 Diabetes Mellitus Based on the Farnesol X Receptor/Ceramide Signaling Pathway Regulating Mitochondrial Metabolism and Endoplasmic Reticulum Stress. Evid Based Complement Alternat Med 2021:9922292 (2021). PubMed: 34422083
- Valuparampil Varghese M et al. Inhibition of Anaplerosis Attenuated Vascular Proliferation in Pulmonary Arterial Hypertension. J Clin Med 9:N/A (2020). PubMed: 32041182
- Varghese MV et al. Antioxidant-Conjugated Peptide Attenuated Metabolic Reprogramming in Pulmonary Hypertension. Antioxidants (Basel) 9:N/A (2020). PubMed: 31991719