概述

  • 产品名称
    PC12核extract裂解物
    参阅全部 PC12 细胞裂解液
  • 常规说明
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.90
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT
    Note: With Protease inhibitors
  • Concentration information loading...
  • 研究领域
  • 背景
    Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.

文献

ab14884 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-4 of 4 Q&A

Answer

Thank you for your reply.

The product ab29545 is a whole cell lysate not a nuclear lysate. The only nuclear lysate we have in stock is the ab14655that you currently have.

If you still wish for me to send you ab29545, the whole cell lysate, please let me know and I will have it sent today. Otherwise, I can refund the cost of ab14655, as it did not produce a signal for you.

I look forward to your reply and resolving this issue for you.

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Answer

Thank you for that information.

I do not think adding 4ul more will increase your signal and since you can only load a maximum of 22ul/well, I would like to send you ab29545 as a free of charge replacement. This HeLa cell lysate is already in sample buffer and is at a higher concentration that the original one sent to you, so you can load more total protein and hopefully see a signal with your 3 antibodies. If this is ok with you, please let me know and I will process the replacement for you.

I look forward to you reply.

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Answer

Thank you for your reply.

I appreciate your patience in this matter, but when you say you loaded 2.5mg/ml of total protein, how many microlitres of lysate is that? I ask, as we send 100ug of total protein at at concentration of 2.5mg/ml (therefore we you should have received 40ul total volume). I just want to make sure that the problem is with the lysate and not that more needs to be used. Although you used both the HeLa and PC12 lysates at the same concentrations, it may be that you need to use more of the HeLa lysate, as you were seeing a signal for Tubulin (the signal was weak, but it was present).

Once again thank you for your patience in this matter, if it does turn out that there is a fault with the lysate, then I would be more than happy to send a new vial.

I look forward to your response.

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Answer

Thank you for contacting Abcam.

I am sorry about the problems you have been having with ab14655, HeLa cell lysate. I was wondering if you could tell me how much total protein you loaded of the cell lysate. I ask as there does seem to be a signal (a weak signal) for Tubulin and I am wondering if you load more of the lysate onto the gel, you would increase the signal for Tubulin and also begin to see a signal for the other antibodies.

If increasing the total amount of protein in the well does not improve the signal, then I would be happy to send you another aliquot of the HeLa lysate.

I look forward to your reply and helping you resolve this issue.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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