Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole mouse brain)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Gly Gel)
Loading amount
20 µg
Specification
Whole mouse brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS + 0.05%Tween
Dilution
1/10000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Cell Signaling Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pierce Pico West ECL
Exposure
50 second(s)
Bands
Specific: 18 kDa
Positive control
1. Antibody only
2. Antibody + 2x (by mass) blocking peptide
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 24 2015