Anti-PBR抗体(ab154878)
Key features and details
- Rabbit polyclonal to PBR
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-PBR抗体
参阅全部 PBR 一抗 -
描述
兔多克隆抗体to PBR -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Horse, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide within Rat PBR aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P16257 -
阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Normal Rat Testes
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab154878于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. -
组织特异性
Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids. -
序列相似性
Belongs to the TspO/BZRP family. -
细胞定位
Mitochondrion membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 706 Human
- Entrez Gene: 12257 Mouse
- Entrez Gene: 24230 Rat
- Omim: 109610 Human
- SwissProt: B1AH88 Human
- SwissProt: P30536 Human
- SwissProt: P50637 Mouse
- SwissProt: P16257 Rat
see all -
别名
- Benzodiazapine receptor (peripheral) antibody
- Benzodiazepine peripheral binding site antibody
- BPBS antibody
see all
图片
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IHC image of TPBR staining in normal Rat testes formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154878, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab154878 被引用在 5 文献中.
- Kitade M et al. Evaluation of (R)-[11C]PK11195 PET/MRI for Spinal Cord-Related Neuropathic Pain in Patients with Cervical Spinal Disorders. J Clin Med 12:N/A (2022). PubMed: 36614916
- Al-Khishman NU et al. TSPO PET detects acute neuroinflammation but not diffuse chronically activated MHCII microglia in the rat. EJNMMI Res 10:113 (2020). PubMed: 32990808
- Koganti PP & Selvaraj V Lack of adrenal TSPO/PBR expression in hamsters reinforces correlation to triglyceride metabolism. J Endocrinol 247:1-10 (2020). PubMed: 32698131
- Huang S et al. Monitoring?the?Progression?of?Chronic Liver Damage in Rats Using [18F]PBR06. Mol Imaging Biol 21:669-675 (2019). PubMed: 30280292
- Owen DR et al. TSPO mutations in rats and a human polymorphism impair the rate of steroid synthesis. Biochem J 474:3985-3999 (2017). WB ; Rat . PubMed: 29074640