Anti-Paxillin (phospho Y31)抗体(ab4832)
Key features and details
- Rabbit polyclonal to Paxillin (phospho Y31)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Paxillin (phospho Y31)抗体
参阅全部 Paxillin 一抗 -
描述
兔多克隆抗体to Paxillin (phospho Y31) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chicken
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免疫原
Synthetic peptide (Human) derived from the region of human paxillin that contains tyrosine 31.
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阳性对照
- ICC: NIH/3T3 (mouse embryo fibroblast cell line), NMµMG (mouse mammary) cells. WB: A431 (human epidermoid carcinoma cell line), HeLa (human epithelial cell line from cervix adenocarcinoma), NIH/3T3 (mouse embryo fibroblast cell line), A549 (human lung carcinoma cell line) cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/250.
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| WB |
1/1000. Predicted molecular weight: 68 kDa.
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| 说明 |
|---|
|
ICC/IF
1/250. |
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WB
1/1000. Predicted molecular weight: 68 kDa. |
靶标
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功能
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). -
序列相似性
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains. -
翻译后修饰
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. -
细胞定位
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. - Information by UniProt
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数据库链接
- Entrez Gene: 395832 Chicken
- Entrez Gene: 5829 Human
- Entrez Gene: 19303 Mouse
- Omim: 602505 Human
- SwissProt: P49024 Chicken
- SwissProt: P49023 Human
- SwissProt: Q8VI36 Mouse
- Unigene: 446336 Human
see all -
别名
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody (ab4832)
Immunocytochemistry/ Immunofluorescence of NIH/3T3 (mouse embryo fibroblast cell line) treated with 50 ng of PDGF for 10 minutes with ab4832 (1/250). A Goat anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugate (1/2000) was used as a secondary (Green). DAPI (Blue) was used as a counter stain. F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel e is untreaded cells and panel f had no primary antibody.
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Western blot - Anti-Paxillin (phospho Y31) antibody (ab4832)All lanes : Anti-Paxillin (phospho Y31) antibody (ab4832)
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate treated for 1 Hr with 100 µM of Pervanadate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated for 20 minutes with 50 ng/mL of TNF alpha
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate treated for 10 minutes with 50 ng/mL of PDGF
Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 8 : A549 (human lung carcinoma cell line) whole cell lysate treated for 20 minutes with 200 nM of PMA
Lysates/proteins at 20 µl per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?ab4832 was diluted between 1/500 and 1/2000.
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Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody (ab4832)Colocalization of phospho Paxillin and actin in TGF-beta treated NMµMG (mouse mammary) cells. Green = actin stress fibers, Orange = Paxillin [pY31], Yellow = colocalization.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab4832 被引用在 5 文献中.
- Li Y et al. Controlling the Multiscale Network Structure of Fibers To Stimulate Wound Matrix Rebuilding by Fibroblast Differentiation. ACS Appl Mater Interfaces 11:28377-28386 (2019). PubMed: 31251577
- Gill MB et al. KSHV-TK is a tyrosine kinase that disrupts focal adhesions and induces Rho-mediated cell contraction. EMBO J 34:448-65 (2015). PubMed: 25471072
- Kawada I et al. Paxillin mutations affect focal adhesions and lead to altered mitochondrial dynamics: relevance to lung cancer. Cancer Biol Ther 14:679-91 (2013). IHC . PubMed: 23792636
- Cassinelli G et al. Inhibition of c-Met and prevention of spontaneous metastatic spreading by the 2-indolinone RPI-1. Mol Cancer Ther 5:2388-97 (2006). WB ; Human . PubMed: 16985073
- Zaffaroni D et al. Met proto-oncogene juxtamembrane rare variations in mouse and humans: differential effects of Arg and Cys alleles on mouse lung tumorigenesis. Oncogene 24:1084-90 (2005). PubMed: 15592501