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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Focal Adhesions

Anti-Paxillin (phospho Y118)抗体(ab4833)

  • Datasheet
  • SDS
Submit a review Submit a question References (6)

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Western blot - Anti-Paxillin (phospho Y118) antibody (ab4833)
  • Western blot - Anti-Paxillin (phospho Y118) antibody (ab4833)

Key features and details

  • Rabbit polyclonal to Paxillin (phospho Y118)
  • Suitable for: WB
  • Reacts with: Mouse, Human, African green monkey
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Paxillin (phospho Y118)抗体
    参阅全部 Paxillin 一抗
  • 描述

    兔多克隆抗体to Paxillin (phospho Y118)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Human, African green monkey
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide corresponding to Human Paxillin (phospho Y118).

  • 阳性对照

    • WB: COS-7, A431, HeLa whole cell lysates
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cancer
    • Signal transduction
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4833于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000. Predicted molecular weight: 68 kDa.
说明
WB
1/1000. Predicted molecular weight: 68 kDa.

靶标

  • 功能

    Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • 序列相似性

    Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • 翻译后修饰

    Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • 细胞定位

    Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Target information above from: UniProt accession P49023 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 395832 Chicken
    • Entrez Gene: 5829 Human
    • Entrez Gene: 19303 Mouse
    • Omim: 602505 Human
    • SwissProt: P49024 Chicken
    • SwissProt: P49023 Human
    • SwissProt: Q8VI36 Mouse
    • Unigene: 446336 Human
    • Unigene: 18714 Mouse
    see all
  • 别名

    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

图片

  • Western blot - Anti-Paxillin (phospho Y118) antibody (ab4833)
    Western blot - Anti-Paxillin (phospho Y118) antibody (ab4833)
    All lanes : Anti-Paxillin (phospho Y118) antibody (ab4833) at 1/500 dilution

    Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 2 : COS-7 whole cell lysate treated with 100 uM Pervanadate for 1 hour
    Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 4 : A431 whole cell lysate treated with 100 uM Pervanadate for 1 hour
    Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 6 : HeLa whole cell lysate treated with 100 uM Pervanadate for 1 hour

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution

    Predicted band size: 68 kDa



    A 66 kDa band corresponding to Paxillin pTyr118 was enhanced across treated cell lines. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

  • Western blot - Anti-Paxillin (phospho Y118) antibody (ab4833)
    Western blot - Anti-Paxillin (phospho Y118) antibody (ab4833)
    All lanes : Anti-Paxillin (phospho Y118) antibody (ab4833) at 0.75 µg/ml (3% BSA-TBST buffer)

    All lanes : NMµMG (mouse mammary) cells transfected with EGFP-tagged paxillin

    Secondary
    All lanes : goat F(ab’)2 anti-rabbit IgG alkaline phosphatase

    Predicted band size: 68 kDa



    Peptide Competition: Extracts were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC.

    Prior incubation with:

    1- no peptide,

    2- the non-phosphopeptide corresponding to the immunogen

    3- a generic phosphotyrosine containing peptide

    4- the phosphopeptide immunogen 

    Bands were detected using the Tropix WesternStar method.

    The data show that only the peptide corresponding to ab4833 blocks the antibody signal, thereby demonstrating the specificity of the antibody. 

实验方案

  • Peptide Competition Experiment
  • Western blotting protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (6)

发表研究结果有使用 ab4833?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab4833 被引用在 6 文献中.

  • Schimmel L  et al. c-Src controls stability of sprouting blood vessels in the developing retina independently of cell-cell adhesion through focal adhesion assembly. Development 147:N/A (2020). PubMed: 32108024
  • Zhang M  et al. Sea cucumber Cucumaria frondosa fucoidan inhibits osteosarcoma adhesion and migration by regulating cytoskeleton remodeling. Oncol Rep 44:469-476 (2020). PubMed: 32467988
  • Wang H  et al. Inhibition of tumor vasculogenic mimicry and prolongation of host survival in highly aggressive gallbladder cancers by norcantharidin via blocking the ephrin type a receptor 2/focal adhesion kinase/paxillin signaling pathway. PLoS One 9:e96982 (2014). WB, ICC/IF ; Mouse . PubMed: 24811250
  • Lange K  et al. Combined lysophosphatidic acid/platelet-derived growth factor signaling triggers glioma cell migration in a tenascin-C microenvironment. Cancer Res 68:6942-52 (2008). WB ; Human . PubMed: 18757408
  • Demo SD  et al. Antitumor activity of PR-171, a novel irreversible inhibitor of the proteasome. Cancer Res 67:6383-91 (2007). PubMed: 17616698
  • Lange K  et al. Endothelin receptor type B counteracts tenascin-C-induced endothelin receptor type A-dependent focal adhesion and actin stress fiber disorganization. Cancer Res 67:6163-73 (2007). WB ; Human . PubMed: 17616673

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