Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK)
Loading amount
10 µg
Specification
MDCK
Gel Running Conditions
Reduced Denaturing (10% tris-glycine gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/2500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk, 0.2% tween-20
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: c.65 kDa
Positive control
All lanes
Additional data
Additional Notes
From left to right - lanes contain MDCK cell lysate (untreated, untransfected whole cell) lysate - lysed in water by homogenisation. Cells treated with concentrations of an in-house synthesised small molecule drug known to cause decrease in levels of paxillin at high concentrations (>100 uM in this cell line). Single band observed at c. 65 KDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 08 2012