重组Anti-Paxillin抗体[Y113] (ab32084)
![Western blot - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.cn/ps/products/32/ab32084/Images/ab32084-467544-anti-paxillin-antibody-y113-westernblot-human-mouse.jpg "Western blot - Anti-Paxillin antibody [Y113] (ab32084)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y113] to Paxillin
- Suitable for: WB, ICC/IF, Flow Cyt (Intra), IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Paxillin抗体[Y113]
参阅全部 Paxillin 一抗 -
描述
兔单克隆抗体[Y113] to Paxillin -
宿主
Rabbit -
特异性
Anti-Paxillin antibody [Y113] (ab32084) recognises Paxillin alpha, beta and gamma isoforms.
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经测试应用
适用于: WB, ICC/IF, Flow Cyt (Intra), IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Dog
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): HeLa cells. ICC: HeLa cells IP: HeLa whole cell lysate WB: HeLa, RAW 264.7 and mouse heart lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解离常数（KD）
KD = 4.17 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y113 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Positive Controls
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Related Products
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Substrate reagent
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32084于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (10) |
1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
For unpurified use at 1/5000 - 1/10000. |
| ICC/IF | (7) |
1/50.
For unpurified use at 1/250. |
| Flow Cyt (Intra) |
1/100.
|
|
| IP | (1) |
1/20.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 68 kDa. For unpurified use at 1/5000 - 1/10000. |
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ICC/IF
1/50. For unpurified use at 1/250. |
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Flow Cyt (Intra)
1/100. |
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IP
1/20. |
靶标
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功能
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). -
序列相似性
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains. -
翻译后修饰
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. -
细胞定位
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. - Information by UniProt
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数据库链接
- Entrez Gene: 5829 Human
- Entrez Gene: 19303 Mouse
- Omim: 602505 Human
- SwissProt: P49023 Human
- SwissProt: Q8VI36 Mouse
- Unigene: 446336 Human
- Unigene: 18714 Mouse
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别名
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
图片
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Western blot - Anti-Paxillin antibody [Y113] (ab32084)All lanes : Anti-Paxillin antibody [Y113] (ab32084) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : Mouse heart lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa -
![Immunocytochemistry - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.cn/ps/products/32/ab32084/Images/ab32084-18-anti-paxillin-antibody-y113-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-Paxillin antibody [Y113] (ab32084)Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Paxillin with purified ab32084 at 1/50 dilution (2.88 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry (Intracellular) - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.cn/ps/products/32/ab32084/Images/ab32084-276941-anti-paxillin-antibody-y113-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Paxillin antibody [Y113] (ab32084)Intracellular Flow Cytometry analysis ofHeLa (human cervix adenocarcinoma) cells labeling with purified ab32084 at 1/100 dilution (10µg/ml) (Red). Cells were fixed with4% paraformaldehydeand permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody.Rabbit monoclonal IgG (Black) (ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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![Immunoprecipitation - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.cn/ps/products/32/ab32084/Images/ab32084-19-anti-paxillin-antibody-y113-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-Paxillin antibody [Y113] (ab32084)Purified ab32084 at 1/20 dilution (0.7µg) immunoprecipitating Paxillin in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg.
Lane 2 (+): ab32084 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32084 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 48 kDa -
![OI-RD Scanning - Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.cn/ps/products/32/ab32084/Images/ab32084-43827-anti-paxillin-antibody-y113.jpg)
OI-RD Scanning - Anti-Paxillin antibody [Y113] (ab32084) -
![Anti-Paxillin antibody [Y113] (ab32084)](https://www.abcam.cn/ps/products/32/ab32084/Images/ab32084-17-benefits-of-recombinant-antibodies.png)
Anti-Paxillin antibody [Y113] (ab32084)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (152)
ab32084 被引用在 152 文献中.
- Kilinc AN et al. Integrin-linked kinase tunes cell-cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFß1. Mol Biol Cell 32:402-412 (2021). PubMed: 33405954
- Koual M et al. Aggressiveness and Metastatic Potential of Breast Cancer Cells Co-Cultured with Preadipocytes and Exposed to an Environmental Pollutant Dioxin: An in Vitro and in Vivo Zebrafish Study. Environ Health Perspect 129:37002 (2021). PubMed: 33683140
- Yeoman B et al. Adhesion strength and contractility enable metastatic cells to become adurotactic. Cell Rep 34:108816 (2021). PubMed: 33691109
- Wang L et al. Combination CTLA-4 immunoglobulin treatment and ultrasound microbubble-mediated exposure improve renal function in a rat model of diabetic nephropathy. Aging (Albany NY) 13:8524-8540 (2021). PubMed: 33714204
- Bain JM et al. Immune cells fold and damage fungal hyphae. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33876755