Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS)
Total protein in input
400 µg
Specification
U2OS
Immuno-precipitation step
Protein A
Other product details
Concentration
5 µg/mg lysate
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA
Additional data
Additional Notes
Lane order: Paxillin IP (lane 1), Con IP (lane 2), 5% Input (lane 3)
Little or no detection of full-length Paxillin in the input lane, however a strong band is observed at 40 kDa in both the input (lane 3) and IP lane (lane 1). The nature of this 40 kDa species is unclear but it has reported using other commercial antibodies to Paxillin (PMID: 11801684), raising the possibility that this product is either an alternatively spliced transcript or a degradation product of Paxillin.
Little or no detection of full-length Paxillin in the input lane, however a strong band is observed at 40 kDa in both the input (lane 3) and IP lane (lane 1). The nature of this 40 kDa species is unclear but it has reported using other commercial antibodies to Paxillin (PMID: 11801684), raising the possibility that this product is either an alternatively spliced transcript or a degradation product of Paxillin.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
提交于 Apr 28 2008