重组Anti-PAX8抗体[EPR23508-20] (ab239363)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23508-20] to PAX8
- Suitable for: WB, IP, IHC-Fr, Flow Cyt (Intra), ChIC/CUT&RUN-seq, ICC/IF, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-PAX8抗体[EPR23508-20]
参阅全部 PAX8 一抗 -
描述
兔单克隆抗体[EPR23508-20] to PAX8 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Fr, Flow Cyt (Intra), ChIC/CUT&RUN-seq, ICC/IF, IHC-Pmore details
不适用于: ChIP -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: NIH:OVCAR-3 and SK-OV-3 whole cell lysates, mouse E18 kidney tissue lysates. IHC-P: Mouse thyroid tissue, human thyroid and ovarian cancer tissue. IHC/FR: Mouse kidney tissue. ICC/IF: NIH:OVCAR-3 and SK-OV-3 whole cells. Flow Cyt (intra): NIH:OVCAR-3 whole cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23508-20 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab239363于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 48 kDa.
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IP |
Use at an assay dependent concentration.
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IHC-Fr |
1/50.
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Flow Cyt (Intra) |
1/500.
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ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
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ICC/IF |
1/100.
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IHC-P |
1/2000.
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说明 |
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WB
1/1000. Predicted molecular weight: 48 kDa. |
IP
Use at an assay dependent concentration. |
IHC-Fr
1/50. |
Flow Cyt (Intra)
1/500. |
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. |
ICC/IF
1/100. |
IHC-P
1/2000. |
靶标
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功能
Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. -
组织特异性
Expressed in the excretory system, thyroid gland and Wilms tumors. -
疾病相关
Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue. -
序列相似性
Contains 1 paired domain. -
发展阶段
In developing excretory system, during thyroid differentiation and in adult thyroid. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7849 Human
- Entrez Gene: 18510 Mouse
- Omim: 167415 Human
- SwissProt: Q06710 Human
- SwissProt: Q00288 Mouse
- Unigene: 469728 Human
- Unigene: 2533 Mouse
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别名
- OTTHUMP00000158659 antibody
- OTTHUMP00000158660 antibody
- OTTHUMP00000203723 antibody
see all
图片
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ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab239363 [EPR23508-20]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab239363 [EPR23508-20]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab239363 [EPR23508-20]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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Anti-PAX8 antibody [EPR23508-20] (ab239363) at 1/1000 dilution + Mouse E18 kidney tissue lysate at 60 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 48 kDaBlocking and diluting buffer and concentration: The molecular weight observed is consistent with what has been described in the literature (PMID: 21602887)
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
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All lanes : Anti-PAX8 antibody [EPR23508-20] (ab239363) at 1/1000 dilution
Lane 1 : SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate
Lane 2 : NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 48 kDaBlocking and diluting buffer and concentration: The antibody detects isoform of PAX8
The molecular weight observed is consistent with what has been described in the literature (PMID: 21602887)
This blot was developed using a higher sensitivity ECL substrate.
Fresh lysate was used in this Lane 2.
Exposure time: Lane 1: 127 seconds Lane 2: 59 seconds
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling PAX8 with ab239363 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on mouse kidney. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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PAX8 was immunoprecipitated from 0.35 mg SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate 10 ug with ab239363 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239363 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate 10 ug
Lane 2: abab239363 IP in SK-OV-3 whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab239363 in SK-OV-3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 49 seconds
This blot was developed using a higher sensitivity ECL substrate.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labelling PAX8 with ab239363 at 1/500 dilution (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-OV-3 (human ovarian cancer epithelial cell) cells labelling PAX8 with ab239363 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in SK-OV-3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labelling PAX8 with ab239363 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (Green). Confocal image showing nuclear staining in NIH:OVCAR-3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling PAX8 with ab239363 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human ovarian cancer (PMID: 21317881). The section was incubated with ab239363 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling PAX8 with ab239363 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human thyroid (PMID: 21317881). The section was incubated with ab239363 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue labeling PAX8 with ab239363 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse thyroid (PMID: 21317881). The section was incubated with ab239363 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab239363 被引用在 1 文献中.
- Ulrich ND et al. Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified using scRNA-seq. Dev Cell 57:914-929.e7 (2022). PubMed: 35320732