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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Parkin / PARK
使用敲除细胞株进行验证重组RabMAb

重组Anti-PARK7/DJ1抗体[EP2815Y] (ab76008)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (26)

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Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Flow Cytometry - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Immunoprecipitation - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Flow Cytometry - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
  • Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2815Y] to PARK7/DJ1
  • Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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概述

  • 产品名称

    Anti-PARK7/DJ1抗体[EP2815Y]
    参阅全部 PARK7/DJ1 一抗
  • 描述

    兔单克隆抗体[EP2815Y] to PARK7/DJ1
  • 宿主

    Rabbit
  • 特异性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Mouse
    WB
    Mouse
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human PARK7/DJ1 aa 1-100 (N terminal).
    Database link: Q99497

  • 阳性对照

    • WB: Jurkat, HeLa, NIH3T3 or 293T cell lysate. Human fetal brain; Human brain nuclear fraction tissue lysate; Mouse brain and Rat brain tissue lysates. IHC-P: Human Lung and Brain tissue. ICC/IF: PANC-1 and Jurkat cell lines. Flow Cyt: HepG2 cells. IP: Mouse brain lysate.
  • 常规说明

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EP2815Y
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Parkin / PARK
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Other
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-PARK7/DJ1 antibody [EP2815Y] (ab203989)
    • Alexa Fluor® 647 Anti-PARK7/DJ1 antibody [EP2815Y] (ab204009)
    • Alexa Fluor® 594 Anti-PARK7/DJ1 antibody [EP2815Y] (ab215102)
    • Anti-PARK7/DJ1 antibody [EP2815Y] - BSA and Azide free (ab218373)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PARK7 (DJ1) knockout HEK-293T cell line (ab266338)
  • KO cell lysates

    • Human PARK7 (DJ1) knockout HEK-293T cell lysate (ab257016)
  • KO cell pellets

    • Human PARK7 (DJ1) knockout HEK293T cell pellet (ab278931)
  • Recombinant Protein

    • Recombinant Human PARK7/DJ1 protein (ab51198)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Mouse
WB
Mouse
Human
应用 Ab评论 说明
WB (2)
1/5000. Predicted molecular weight: 20 kDa.

For unpurified use at 1/10000 - 1/20000.

IP
1/20.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/250 - 1/500.

Flow Cyt
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100

ICC/IF (1)
1/50 - 1/500.
说明
WB
1/5000. Predicted molecular weight: 20 kDa.

For unpurified use at 1/10000 - 1/20000.

IP
1/20.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/250 - 1/500.

Flow Cyt
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100

ICC/IF
1/50 - 1/500.

靶标

  • 功能

    Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone.
  • 组织特异性

    Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain. Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.
  • 疾病相关

    Defects in PARK7 are the cause of Parkinson disease type 7 (PARK7) [MIM:606324]. A neurodegenerative disorder characterized by resting tremor, postural tremor, bradykinesia, muscular rigidity, anxiety and psychotic episodes. PARK7 has onset before 40 years, slow progression and initial good response to levodopa. Some patients may show traits reminiscent of amyotrophic lateral sclerosis-parkinsonism/dementia complex (Guam disease).
  • 序列相似性

    Belongs to the peptidase C56 family.
  • 翻译后修饰

    Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
    Cys-106 is easily oxidized to sulfinic acid.
    Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.
  • 细胞定位

    Cytoplasm. Nucleus. Mitochondrion. Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients.
  • Target information above from: UniProt accession Q99497 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 11315 Human
    • Entrez Gene: 57320 Mouse
    • Entrez Gene: 117287 Rat
    • Omim: 602533 Human
    • SwissProt: Q99497 Human
    • SwissProt: Q99LX0 Mouse
    • SwissProt: O88767 Rat
    • Unigene: 419640 Human
    • Unigene: 277349 Mouse
    • Unigene: 30105 Rat
    see all
  • 别名

    • CAP1 antibody
    • DJ-1 antibody
    • DJ1 antibody
    • DJ1 protein antibody
    • Epididymis secretory sperm binding protein Li 67p antibody
    • FLJ27376 antibody
    • FLJ34360 antibody
    • FLJ92274 antibody
    • HEL S 67p antibody
    • Oncogene DJ1 antibody
    • OTTHUMP00000001348 antibody
    • OTTHUMP00000001349 antibody
    • OTTHUMP00000001350 antibody
    • OTTHUMP00000001351 antibody
    • PARK7 antibody
    • PARK7_HUMAN antibody
    • Parkinson disease (autosomal recessive, early onset) 7 antibody
    • Parkinson disease protein 7 antibody
    • Parkinson protein 7 antibody
    • Protein DJ-1 antibody
    • SP22 antibody
    see all

图片

  • Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    All lanes : Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : PARK7 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : Human brain nuclear fraction tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 20 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab76008 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab76008 Anti-PARK7/DJ1 antibody [EP2815Y] was shown to specifically react with PARK7/DJ1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266338 (knockout cell lysate ab257016) was used. Wild-type and PARK7/DJ1 knockout samples were subjected to SDS-PAGE. ab76008 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling PARK7/DJ1 with Purified ab76008 at 1:500 dilution (0.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling PARK7/DJ1 with Purified ab76008 at 1:1000 dilution (0.11 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Flow Cytometry - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling PARK7/DJ1 with Purified ab76008 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PARK7/DJ1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Human brain tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab76008 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab76008 was shown to specifically react with PARK/DJ1 in wild-type HAP1 cells. No band was observed when PARK/DJ1 knockout samples were used. Wild-type and PARK/DJ1 knockout samples were subjected to SDS-PAGE. ab76008 and ab8245 (loading control to GAPDH) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Immunoprecipitation - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Immunoprecipitation - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

    ab76008 (purified) at 1:20 dilution (0.5µg) immunoprecipitating PARK7/DJ1 in Mouse brain lysate.
    Lane 1 (input): Mouse brain lysate 10µg
    Lane 2 (+): ab76008 & Mouse brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76008 in Mouse brain lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    All lanes : Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008) at 1/5000 dilution (Purified) + Human fetal brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 20 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling PARK7/DJ1 with purified ab76008 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

    This image was generated using the unpurified version of the product. 

  • Flow Cytometry - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Flow Cytometry - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

    Overlay histogram showing HepG2 cells stained with ab76008 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76008, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the unpurified version of the product. 

  • Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    All lanes : Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008) at 1/20000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : NIH3T3 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?



    This image was generated using the unpurified version of the product. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

    ab76008, at 1/250 dilution, staining PARK7/DJ1 in human brain by immunohistochemistry using paraffin-embedded tissue.

    This image was generated using the unpurified version of the product. 

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)
    Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

实验方案

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (26)

发表研究结果有使用 ab76008?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab76008 被引用在 26 文献中.

  • Schultrich K  et al. Effects of 2-MCPD on oxidative stress in different organs of male mice. Food Chem Toxicol 142:111459 (2020). PubMed: 32474023
  • Ji YJ  et al. Down-regulation of DJ-1 Augments Neuroinflammation via Nrf2/Trx1/NLRP3 Axis in MPTP-induced Parkinson's Disease Mouse Model. Neuroscience N/A:N/A (2020). PubMed: 32526245
  • Kooij R  et al. Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-Terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos. J Am Chem Soc 142:16825-16841 (2020). PubMed: 32886496
  • Xu W  et al. Sodium Benzoate Attenuates Secondary Brain Injury by Inhibiting Neuronal Apoptosis and Reducing Mitochondria-Mediated Oxidative Stress in a Rat Model of Intracerebral Hemorrhage: Possible Involvement of DJ-1/Akt/IKK/NF?B Pathway. Front Mol Neurosci 12:105 (2019). PubMed: 31114478
  • Gao L  et al. Natrium Benzoate Alleviates Neuronal Apoptosis via the DJ-1-Related Anti-oxidative Stress Pathway Involving Akt Phosphorylation in a Rat Model of Traumatic Spinal Cord Injury. Front Mol Neurosci 12:42 (2019). PubMed: 30853891
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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-PARK7/DJ1 antibody [EP2815Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (induced pluripotent stem cell)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% gradient)
Loading amount
20 µg
Specification
induced pluripotent stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Abcam user community

Verified customer

提交于 Aug 16 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-PARK7/DJ1 antibody [EP2815Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (induced pluripotent stem cell)
Permeabilization
Yes - 0.3% Triton X-100
Specification
induced pluripotent stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Aug 16 2019

Question

I am interested in purchasing the DJ-1 antibody against the amino acid (178-189). Iwould also like toknow if you have antibody available for the active form of DJ-1 ( 1-174)?

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Abcam community

Verified customer

Asked on Feb 14 2012

Answer

Thank you for contacting Abcam.
I believe that ab76008 would wwork for you as the peptide for this antibody maps between amino acids ********* of human DJ-1 (UniProt: Q99497).
Please let me know if you have any questions.

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Abcam Scientific Support

回复于 Feb 14 2012

Western blot abreview for Anti-PARK7/DJ1 antibody [EP2815Y]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Treatment
RIPA buffer, 4x Laemmli buffer
Gel Running Conditions
Reduced Denaturing (12% PAGE)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

提交于 Oct 27 2010

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