Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine gel)
Sample
Human Cell lysate - whole cell (SHSY5Y, N2a, HT22)
Specification
SHSY5Y, N2a, HT22
Blocking step
Odyssey blocking soln as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 37°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking soln with 0.1% tween 20
Dilution
1/750
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680
Dilution
1/10000
Detection
Detection method
LICOR
Bands
Specific: 98 kDa
Exposure
45 minute(s) and 0 second(s)
Additional data
Abcam response
Thank you very much for your valuable feedback. We have only tested the antibody with mouse lysates, so it's possible that it does not strongly react with human samples. To improve the signal, we suggest using 3% milk to block and incubating with a 1:500 dilution of the antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Kalpita Banerjee
Verified customer
提交于 Sep 05 2013