Anti-PAR6抗体(ab6022)

Submit a review Q&A (3)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)

Key features and details

  • Goat polyclonal to PAR6
  • Suitable for: IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-PAR6抗体
    参阅全部 PAR6 一抗

  • 描述

    山羊多克隆抗体to PAR6

  • 宿主

    Goat

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Peptide with sequence

    GSRIRGDGSGFSL

    , from C Terminus of the protein sequence according to NP_058644
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    GenBank Accession Number – NP_058644.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab6022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明
    Peptide ELISA: Antibody detection limit dilution 1:8,000.
    IHC-P: Use at a concentration of 5 - 10 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能

      Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

    • 组织特异性

      Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.

    • 序列相似性

      Belongs to the PAR6 family.
      Contains 1 OPR domain.
      Contains 1 PDZ (DHR) domain.
      Contains 1 pseudo-CRIB domain.

    • 结构域

      The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
      The OPR domain mediates interactions with MAP2K5.
      The PDZ domain mediates the interaction with CRB3.

    • 细胞定位

      Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
    • Information by UniProt

    • 数据库链接

      • 别名

        • 0710008C04Rik antibody
        • 2610010A15Rik antibody
        • Par 6 partitioning defective 6 C elegans homolog alpha antibody
        • Par 6 partitioning defective 6 homolog alpha antibody
        • Par 6 partitioning defective 6 homolog alpha C elegans antibody
        • PAR 6A antibody
        • PAR-6 alpha antibody
        • PAR-6 antibody
        • par-6 family cell polarity regulator alpha antibody
        • PAR-6A antibody
        • PAR6 antibody
        • PAR6A_HUMAN antibody
        • PAR6alpha antibody
        • PAR6C antibody
        • Pard6a antibody
        • Partitioning defective 6 homolog alpha antibody
        • partitioning-defective protein 6 antibody
        • partitioning-defective protein 6, C. elegans, homolog of, alpha antibody
        • Tax interacting protein 40 antibody
        • Tax interaction protein 40 antibody
        • TAX40 antibody
        • TIP 40 antibody
        • TIP-40 antibody
        • TIP40 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)
        Ab6022 (10ug/ml) staining human PAR6 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

        Ab6022 shows strong cytoplasmic staining in rare cells of intralobular ducts.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)

        ab6022 at 5ug/ml staining PAR6 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

      文献 (1)

      发表研究结果有使用 ab6022？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6022 被引用在 1 文献中.

      • Wu M  et al. Epicardial Spindle Orientation Controls Cell Entry into the Myocardium. Dev Cell 19:114-125 (2010). IHC-P ; Mouse . PubMed: 20643355

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Question

      Dear,
      I'm a PhD student working at EMBL (European Molecular Biology Laboratory).
      I'm looking for an antibody anti-PAR6 (Partion Defective 6). I found in
      lecterature that abcam was selling an antibody able to recognize all the 3
      isoform of the protein catalog number ab45394. Unfortunately this product
      is not anymore available. It is something temporally or will be not sell
      anymore? I look the other antibody available but in the datasheet is not
      specificated if they react with all the 3 isoform of the protein. There is
      a specific product in substitution of ab45394?
      thank you in advance.
      Kinds Regards

      Read More
      Answer

      Thank you for your inquiry.

      Unfortunately, we only have one antibody in our catalog at the moment that is tested to work with mouse. The immunogen of does not show a high homology with mouse but the antibody was used successfully in the following publication:

      PubMed: 20643355, Figure 7 IHC.

      The antibody most likely binds to the last coupleof amino acids in the immunogen. This would also mean that all three isoform should be recognized.

      But this has not been experimentally tested and we do not know yetthat this antibody is good for mouse under all circumstances and applications in all mouse samples.

      I am sorry I did not have positive and straight forward information for you on this occasion and hope this information is nevertheless helpful to you.

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      Question

      Status of: #****** : received in late february #**** : Unfortunately ab45394 is now unpublished. studied species : Mouse application : IF, WB, IP

      Read More
      Answer

      Comme convenu, la commande numéro ****** (une unité gratuite de ab6022) a été expédiée aujourd'hui. Vous devriez recevoir le produit demain. J'espère que l'anti-PAR6 ab6022 fonctionnera chez la souris. Nous serions ravis si vous pouviez nous envoyer une Abreview avec votre feedback sur l'utilisation de ab6022 chez la souris. Le lien pour soumettre cette Abreview est : https://www.abcam.com/PAR6-antibody-ab6022-reviews.html N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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      Question

      We have injected the homogenized tumor tissue isolated from human lung into the armpit of mice. After certain time the neoplasm is formed in the armpit. Now we want to know the nature of the neoplasm that is originally from human lung tumor or is a new tumor from mouse. We would like to check the nature of the neoplasm by IHC (florescence). Could you please tell us which antibody that can specifically recognize human tissue (tumor) or mouse tissue (tumor) ? We hope that antibody does not cross-react on human or mouse. Also, the host of antibody is not form mouse.

      Read More
      Answer

      Thank you for contacting us. I have heard of people using anti-vimentin clone V9, to detect human tissue implanted in mice. However, this is a mouse monoclonal and our version, ab8069 is unconjugated. We do not have a version conjugated to a fluorochrome, enzyme, or other detector. An anti-mouse IgG secondary may give some staining of endogenous IgG, as you are probably aware. For detecting mouse tissue without detecting human, consider ab15630, with is also available biotinylated as ab15631 and FITC-conjugated as ab15638: https://www.abcam.com/index.html?datasheet=15630 The ab15630 and ab15631 have been shown to work on frozen tissue, but will not work on paraffin-embedded tissue. Ab15638 has only been tested for flow cytometry so we canot guarantee it will work for IHC. Antibody ab6022 has not been tested on mouse tissue. An alignment of the immunogenic peptide with the mouse protein shows that it is 69% identical; it is difficult to predict from this if the antibody will or will not cross-react with the mouse protein. The alignment can be found at this address for the next 24 hours: http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20070510-00025988&poll=yes I hope this helps; please do not hesitate to contact us if you have any questions.

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