Anti-PAR6抗体(ab6022)
Key features and details
- Goat polyclonal to PAR6
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PAR6抗体
参阅全部 PAR6 一抗 -
描述
山羊多克隆抗体to PAR6 -
宿主
Goat -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Peptide with sequence
GSRIRGDGSGFSL
, from C Terminus of the protein sequence according to NP_058644 -
常规说明
GenBank Accession Number – NP_058644.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab6022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
IHC-P: Use at a concentration of 5 - 10 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. -
组织特异性
Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta. -
序列相似性
Belongs to the PAR6 family.
Contains 1 OPR domain.
Contains 1 PDZ (DHR) domain.
Contains 1 pseudo-CRIB domain. -
结构域
The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
The OPR domain mediates interactions with MAP2K5.
The PDZ domain mediates the interaction with CRB3. -
细胞定位
Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions. - Information by UniProt
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数据库链接
- Entrez Gene: 50855 Human
- Entrez Gene: 56513 Mouse
- Omim: 607484 Human
- SwissProt: Q9NPB6 Human
- SwissProt: Q9Z101 Mouse
- Unigene: 112933 Human
- Unigene: 472753 Mouse
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别名
- 0710008C04Rik antibody
- 2610010A15Rik antibody
- Par 6 partitioning defective 6 C elegans homolog alpha antibody
see all
图片
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Ab6022 (10ug/ml) staining human PAR6 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Ab6022 shows strong cytoplasmic staining in rare cells of intralobular ducts.
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ab6022 at 5ug/ml staining PAR6 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
数据表及文件
文献 (1)
ab6022 被引用在 1 文献中.
- Wu M et al. Epicardial Spindle Orientation Controls Cell Entry into the Myocardium. Dev Cell 19:114-125 (2010). IHC-P ; Mouse . PubMed: 20643355