Anti-PAR1/Thrombin Receptor抗体(ab32611)
Key features and details
- Rabbit polyclonal to PAR1/Thrombin Receptor
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PAR1/Thrombin Receptor抗体
参阅全部 PAR1/Thrombin Receptor 一抗 -
描述
兔多克隆抗体to PAR1/Thrombin Receptor -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Baboon -
免疫原
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阳性对照
- KNRK cells, Pancreatic tissue.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32611于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. -
组织特异性
Platelets and vascular endothelial cells. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2149 Human
- Omim: 187930 Human
- SwissProt: P56488 Baboon
- SwissProt: P25116 Human
- Unigene: 482562 Human
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别名
- CF2R antibody
- Coagulation factor II (thrombin) receptor antibody
- Coagulation factor II receptor antibody
see all
图片
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Anti-PAR1/Thrombin Receptor antibody (ab32611) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesPAR1/Thrombin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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This image shows human pancreas stained with ab32611.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab32611 被引用在 13 文献中.
- Guan Y et al. Protease-Activated Receptor 1 Contributes to Microcirculation Failure and Tubular Damage in Renal Ischemia-Reperfusion Injury in Mice. Biomed Res Int 2021:6665714 (2021). PubMed: 33681367
- Dekker NAM et al. Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental cardiopulmonary bypass. Intensive Care Med Exp 9:30 (2021). PubMed: 34169407
- Gerasimov A et al. Markers for neural degeneration and regeneration: novel highly sensitive methods for the measurement of thrombin and activated protein C in human cerebrospinal fluid. Neural Regen Res 16:2086-2092 (2021). PubMed: 33642398
- Perry EA et al. Tetracyclines promote survival and fitness in mitochondrial disease models. Nat Metab 3:33-42 (2021). PubMed: 33462515
- Shavit-Stein E et al. Brain Protease Activated Receptor 1 Pathway: A Therapeutic Target in the Superoxide Dismutase 1 (SOD1) Mouse Model of Amyotrophic Lateral Sclerosis. Int J Mol Sci 21:N/A (2020). PubMed: 32408605