兔多克隆抗体to Papillomavirus Regulatory Factor 1 / HDBP2
Synthetic peptide (Human) from the center region of Papillomavirus Regulatory Factor 1/ HDBP2, conjugated to KLH.
- Y79 cell lysate
Human hepatocellular carcinoma tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/100. Predicted molecular weight: 55 kDa.
||1/50 - 1/100.
Plays a role in papillomavirus genes transcription.
Contains 1 C2H2-type zinc finger.
Cytoplasm. Nucleus. May shuttle between nucleus and cytoplasm.
Information by UniProt
- DKFZp434K1210 antibody
- HD gene regulatory region binding protein 2 antibody
- HD gene regulatory region-binding protein 2 antibody
Western blot - Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727)This image is courtesy of an anonymous Abreview
All lanes :
Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727) at 1/100 dilutionAll lanes :
Human renal cancer whole cell lysate
Lysates/proteins at 50 µg per lane.SecondaryAll lanes :
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186692
Performed under reducing conditions.Predicted band size:
55 kDaObserved band size:
55-60 kDa (why is the actual band size different from the predicted?
)Additional bands at:
25 kDa (possible non-specific binding), 37 kDa (possible non-specific binding)Exposure time:
Western blot - Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727)
Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727) at 1/60 dilution + Y79 cell lysate at 35 µg
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 37 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue labelled with ab75727 at 1/50 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
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